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目的：利用MMTV-erbB-2 转基因小鼠，探讨食物中大豆异黄酮对MMTV-erbB-2 转基因小鼠乳腺肿瘤发生发展的影响。方法：选择健康雌性MMTV-erbB-2 转基因小鼠60 只，随机分为实验组（自鼠龄四周起喂养含有大豆异黄酮的豆饲料和对照组（喂养不含大豆异黄酮的普通饲料）。观察两组小鼠生长情况，观察各组小鼠乳腺肿瘤的发病率和潜伏期、记录肿瘤生长情况，并通过 HE 染色观察其病理类型，免疫组织化学染色SP 法检测各组小鼠乳腺癌组织及正常乳腺组织中MMP-2 和TIMP-2 的表达并分析其关系。结果：豆饲料干预组，普通饲料干预组小鼠乳腺肿瘤的发瘤率分别为36.7 % ，66.7 %，豆饲料干预组小鼠乳腺肿瘤发瘤率与对照饲料干预组相比明显降低，差异有统计学意义（P<0.05）。小鼠肿瘤多生长在第2-3 对乳腺上，两组小鼠乳腺肿瘤最大平均直径及潜伏期相比较差异无统计学意义。两实验组小鼠乳腺肿瘤组织经HE 染色后全部确定为乳腺癌组织。两实验组小鼠乳腺肿瘤组织中MMP-2 和TIMP-2 表达均高于正常乳腺组织，差异有统计学意义（P<0.05），MMP-2 和TIMP-2 在乳腺肿瘤组织中表达呈负相关，在正常乳腺组织中表达无相关性。MMP-2 在豆饲料干预组，普通饲料干预组小鼠乳腺肿瘤组织中的阳性率分别为 83.3 %，73.9 % ，各实验组阳性率相比较差异无统计学意义（P=0.888）；TIMP-2 在豆饲料干预组，普通饲料干预组小鼠乳腺肿瘤组织中的阳性率分别为33.3 %，43.5 %，各实验组阳性率相比较差异无统计学意义。结论：大豆异黄酮能抑制MMTV-erbB-2 转基因小鼠乳腺肿瘤的发生，但其对小鼠乳腺肿瘤的作用与MMP-2 及TIMP-2 的表达无明显相关，具体机制尚待进一步研究。

英文摘要:

Objective: This experiment using the MMTV-erbB-2 transgenic mice, discusses the influence of the soybean isoflavone in food to the occurrence and development of MMTV-erbB-2 transgenic mice 's breast tumor. Methods: 60 female MMTV-erbB-2 transgenic mice were selected and divided into two groups: Bean feed intervention group: treated the mice with bean feed contain soybean isoflavones since four weeks after born; Ordinary feed intervention group: treated the mice with ordinary feed contains no soybean isoflavones. Observed the growth and the development of the mice, observed the incidence of breast cancer of the mice, the tumor latent period, record the growth situation of the tumor, made HE staining to observe the pathological type, using the immunohistochemistry staining to detected the MMP-2 and TIMP-2's expression in both normal breast tissue and breast tumor tissue of each group, and analyzed their relationship. Results: The incidence of breast tumor in bean feed intervention group and common feed intervention group were 36.7 %, 66.7 % . The incidence of breast tumor in bean feed intervention group were significantly reduced compared with the controlled feed intervention group, the difference had statistically significant (P<0.05). Most of the tumors located on the second and the third mammary gland, the maximum average diameter of the tumor in each groups compared to each other had no statistically significant difference. All the breast tumor tissues were determined to be breast cancer organizations by H E staining. The MMP-2 and TIMP-2's expression in breast tumor tissues were both higher than in normal breast tissues, the difference was statistically significant (P<0.05), the MMP-2 and TIMP-2's expression in breast tumor tissues had a negative correlation, and their expressions in normal breast tissue were unrelated. The positive rate of MMP-2 in bean feed intervention group and common feed intervention group's breast tumor tissue were 83.3 % and 73.9 % , the difference compared with each other had no statistically significant (P = 0.888). The positive rate of TIMP-2 in beans feed intervention group and common feed intervention group's breast tumor tissue were 33.3 % and 43.5 %, the difference compared with each other had no statistically significant. Conclusion: Soybean isoflavone can restrain the breast tumor on MMTV-erbB-2 transgenic mice, but it have no obvious relation with the expression of MMP-2 and TIMP-2 in mice's breast tumor, and the specific mechanism needto be studied further.

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