| 何仁忠1,2 朱黎明1△ 符代炎1 霍琼芬2 黄进2 李海银2.骨髓淋巴细胞的分离[J].,2012,12(16):3037-3039 |
| 骨髓淋巴细胞的分离 |
| Separation of Bone Marrow Lymphocytes |
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| DOI： |
| 中文关键词: 骨髓淋巴细胞 分离 炎症性疾病 |
| 英文关键词: Bone marrow lymphocytes Separation Inflammation disease |
| 基金项目:湖南省自然科学基金（09JJ5019） |
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| 中文摘要: |
| 目的：探讨骨髓淋巴细胞的分离方法。方法：对18 例疑似血液病患者，分别抽取骨髓0.2ml，参照淋巴细胞分离步骤来分离
骨髓淋巴细胞，并且对骨髓细胞进行活力检测，分别观察骨髓淋巴细胞的形态及计算骨髓淋巴细胞的活力。结果：骨髓淋巴细胞
外形呈圆形或椭圆形、边缘整齐，胞浆量很少，似裸核，胞质如可见，呈淡蓝色，一般无颗粒；胞核圆形或有小切迹，染色质聚集成
大块状，结构紧密，结块边缘不清楚，染紫红色；镜下观察发现骨髓淋巴细胞的活力>95 %。结论：Ficoll 分离液可以对骨髓淋巴细
胞进行分离，将为成熟淋巴细胞的研究以及其在炎症性疾病中的发病机制及治疗提供一个新的思路。 |
| 英文摘要: |
| Objective: To investigate the method to isolate the bone marrow cell. Methods: 0.2 ml bone marrow was extracted
from 18 cases of suspected blood diseases. Bone marrow cell was isolated according to the separation lymphocytes steps. Dynamic testing
was done to observe the form of bone marrow cells and calculate the vitality of bone marrow lymphocytes. Results: Lymphocytes
from bone marrow cells were round or oval shape, neat edge, with small amount of cytoplasm, like the bare nucleus, cytoplasm, such as
visible, pale blue, usually without granules; Nucleus was round or with a small notch, staining chromatin aggregation into big blocky,
compact structure, indistinct agglomeration edge, dyed by aubergine; the vitality of the bone marrow lymphocytes were above 95 % as
observed by microscope. Conclusion: The bone marrow lymphocyte can be separated by Ficoll separation medium. This provides a new
way for experimental study on mature Lymphocyte and the pathogenesis and treatment for inflammatory diseases. |
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