| 谈高 曾斌 熊丹 阳琼 薛义炯 廖罗罡睿.5- 脱氧杂氮胞苷对人肝癌细胞HepG2 增殖及beclin1 表达的影响[J].,2012,12(14):2654-2657 |
| 5- 脱氧杂氮胞苷对人肝癌细胞HepG2 增殖及beclin1 表达的影响 |
| Effect of 5-aza-2'-Deoxycity Dine on Proliferation of HepG2 Cellsand Expression of Beclin1 |
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| DOI： |
| 中文关键词: 5- 脱氧杂氮胞苷 肝癌细胞 HepG2 beclin1 基因 |
| 英文关键词: 5-aza-2'-deoxycitydine Hepatoma cells HepG2 Beclin1 gene |
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| 中文摘要: |
| 目的：观察5- 脱氧杂氮胞苷(5-aza-CdR)对HepG2 细胞生长抑制及beclin1 表达的影响, 以探讨其抗肿瘤发生的潜在机制。
方法：采用四甲基偶氮唑盐(MTT)法检测5-aza-CdR 对HepG2 细胞的生长抑制；用相差显微镜观察不同药物浓度下不同时间段
的肝癌细胞形态学改变；采用RT-PCR 法和Western blot 法检测5-Aza-CdR 对抑癌基因beclin1 的mRNA 和蛋白表达的影响。结
果：5-aza-CdR 可抑制HepG2 细胞生长, 呈剂量依赖性，并上调beclin1 的mRNA 和蛋白的表达。结果：显示102.4umol/L5-aza-CdR
作用72 小时细胞增殖抑制率最高，可达(84.3±3.31)%,beclin1 的mRNA 和蛋白表达上调最明显，与对照组相比差异有统计学
意义。结论：5-aza-CdR 可抑制HepG2 细胞增殖, 其机制可能是通过恢复某些抑癌基因的表达, 上调beclin1 的mRNA 和蛋白表
达。 |
| 英文摘要: |
| Objective: To observe the effect of 5-aza-2'-deoxycitydine on cell growth of HepG2 and the expression of beclin1,and
to investigate the potential mechanism of its anti tumorigenesis. Methods: Cell growth inhibition was assayed by MTT method; Morphological
changes of HepG2 were observed by phase contrast microscopy under different drug concentrations in different time periods; To
detect the effect of 5-aza-CdR on expression of mRNA and protein in tumor suppressor gene beclin1 by RT-PCR and Western blot.
Results: 5-aza-2'-deoxycitydine could inhibit HepG2 cell growth in a concentration-dependent manner, and increased beclin1 expression
of mRNA and protein. The results showed that after 72 hours in 102.4umol/L5-aza-CdR intervention the rate of cell proliferation
inhibition reached its maximum, up to (84.3±3.31)%, beclin1 expression of mRNA and protein increased most significantly. Difference
was statistically significant compared with the control group. Conclusions: 5-aza-2'-deoxycitydine can inhibit the proliferation of HepG2
cells, and the mechanism may be that 5-aza-2'-deoxycitydine restores some tumor suppressor genes expression and increases the expression
of beclin1. |
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