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目的：探讨不同剂量环磷酰胺对小鼠肿瘤成模情况的影响，寻找一种简单、有效的建立肿瘤模型的预处理方法。方法：给予 BALB/c 裸鼠腹腔注射不同剂量环磷酰胺，72 小时后再给予小鼠皮下接种淋巴瘤细胞，观察预处理前后小鼠外周血白细胞数量及体重变化情况，以及肿瘤成模率、急性死亡率等。结果：①组1（NS 对照组）、组2（100mg/kg×1d）、组3（125mg/kg×1d）、组4 （75mg/kg×2d）预处理后体重较处理前无显著性变化，亦无急性死亡情况发生；而组5（125mg/kg×2d）、组6（200mg/kg×2d）、组7 （125mg/kg×3d）、组8（250mg/kg×3d）小鼠体重较预处理前明显减轻，且急性死亡率依次为30%、58.3%、50%、75%；②组1 和组2 小鼠预处理后72 小时外周血白细胞数较处理前无明显差异，同时均未成模；而组3、组4、组5、组6、组7、组8 小鼠白细胞较预处理前均显著下降，成模率依次为20%、83.3%、60%、33.3%、50%、25%。结论：使用环磷酰胺75mg/kg 连续2 天腹腔注射的预处理方案，操作简单，成本低廉，通过观察外周血白细胞数和小鼠体重水平等指标即可初步判断建模情况，同时肿瘤成模率高，毒副作用小，是理想的预处理方案。

英文摘要:

Objective: To investigate the effects of different doses of cyclophosphamide on the tumor implantation and growth rate in immunodeficient mice models. Methods: Different doses of cyclophosphamide were given to BALB/c nude mice, and then human lymphoma cells were inoculated for 72 hours. The effect of pretreating mice with cyclophosphamide was compared, as well as the tumor implantation and growth rate. Results:①The mice in group 1, group 2, group 3 and group 4 had no changes in body weight and the acute death rate after treated with cyclophosphamide. Mice in group 5, group 6, group 7 and group 8 lost their weight significantly after treated with cyclophosphamide. The acute death rate of each group was 30%, 58.3%, 50%, 75%. ②The mice in group 1 and group 2 had no changes in WBC count, and no lymphoma model developed either. The WBC count was significantly decreased in mice of group 3, group 4, group 5, group 6, group 7 and group 8, and the tumor implantation rate of each group was 20%, 83.3%, 60%, 33.3%, 50%, 25%. Conclusion: The mouse cancer models can be established by intraperitoneal injection of cyclophosphamide, and the dose of 75mg/kg for 2 days was the best dose for establishing the model.

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