| 刘红姜鸿岳华△ 陆晨刘凯.联合标记物检测在重症感染中合并急性肾损伤的诊断价值[J].,2012,12(13):2535-2538 |
| 联合标记物检测在重症感染中合并急性肾损伤的诊断价值 |
| Diagnostic Value of Combined Detection of Markers in SevereInfection with Acute Kidney Injury |
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| DOI： |
| 中文关键词: NGAL Kim-1 NAG mALB 重症感染 急性肾损伤 |
| 英文关键词: NGAL Kim-1 NAG mALB Severe infection Acute kidney injury |
| 基金项目:新疆维吾尔自治区人民医院课题资助（20100118） |
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| 中文摘要: |
| 目的：探讨尿中性粒细胞明胶酶相关载脂蛋白（neutrophil gelatinase-associated lipocalin,NGAL）、尿肾损伤分子-1（kidney
injury molecule-1,Kim-1）、尿N- 乙酰-β-D- 氨基葡萄糖苷酶(N-acecyl-β-D-glucosaminidase,NAG)、尿微量白蛋白（mALB）在重症
感染中合并急性肾损伤的敏感性及临床价值。方法：回顾分析60 例在新疆自治区人民医院ICU 住院的重症感染合并急性肾损伤
(AKI)患者的尿NGAL、Kim-1、NAG 及mALB 的变化情况。健康体检者20 例为对照组。尿NGAL、Kim-1、mALB 测定采用酶联免
疫法（ELISA）检测，尿NAG 测定采用对硝基苯酚（PNP）比色法检测，并以ROC 曲线分析其敏感性。结果：AKI 组患者尿液中的
NGAL、Kim-1、NAG、mALB 的测定浓度明显高于对照组，差异具有统计学意义（P＜0.001）,通过ROC 曲线、诊断试验结果显示：
尿NGAL、Kim-1 曲线下面积分别为0.986、0.956，95%可信区间分别是0.968～1.004、0.910～1.001，较尿NAG、mALB 更具有敏
感性（P＜0.001）。结论：尿NGAL、尿Kim-1 的浓度检测对重症感染合并急性肾损伤的诊断更具有敏感性，与NAG、mALB 联合检
测有助于急性肾损伤的早期监测，对预防急性肾损伤的发生、发展具有重要的临床价值。 |
| 英文摘要: |
| Objective: To explore the sensitivity and clinical value of urine neutrophil gelatinase-associated lipocalin（NGAL）,
kidney injury molecule-1(Kim-1）, urine N-acetyl-beta-D-glucosaminidase(NAG) and urine microalbumin(mALB) in patients of severe
infection with acute kidney injury. Methods: Retrospectively analysis the changes of urine NGAL, urine Kim-1, urine NAG and urine
mALB in 60 cases of severe infection with acute kidney injury who hospitalized in ICU. 20 healthy subjects as control group. The urine
NGAL, urine Kim-1 and urine mALB were respectively determined by enzyme linked immunosorbent assay (ELISA),Urine NAG was
measured by p-nitropheno (PNP) colorimetric assay, the sensitivity was analyzed by ROC curves. Results: Urine NGAL, Urine Kim-1,
urine NAG and urine mALB were significantly higher in AKI group, compared with control group, there were statistically significances
between AKI group and control group (P＜0.001). Through the ROC curve and diagnostic test, the results showed that, the area under the
curve of urine NGAL and urine Kim-1 were 0.986, 0.956. the 95% confidence interval of urine NGAL and urine Kim-1 were
0.968-1.004, 0.910-1.001.urine NGAL and urine Kim-1were more sensitive than urine NAG and urine mALB (P＜0.001). Conclusions：
Urine NGAL and urine Kim-1 had more diagnosis sensitivity for patients of severe infection with acute kidney injury. Combined
detection of urine NGAL, urine Kim-1, urine NAG and urine mALB were helpful for early diagnosis of acute kidney injury, and had
important clinical value on the prevention in pathogenesis and development of acute kidney injury. |
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