| 殷继鹏1 窦维佳1 李明1 惠晓丽2 胡皓1 幺立萍1 聂勇战1 吴开春1△.胃癌血管靶向肽GX2 对胃癌血管内皮细胞靶向性的鉴定[J].,2012,12(13):2470-2473 |
| 胃癌血管靶向肽GX2 对胃癌血管内皮细胞靶向性的鉴定 |
| Identification of Targeting Ability of GX2 Homing to Gastric CancerVasculature |
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| DOI： |
| 中文关键词: 肿瘤靶向 GX2 胃癌 人脐静脉内皮细胞 |
| 英文关键词: Tumor targeting GX2 Gastric cancer HUVEC |
| 基金项目:国家自然科学基金重大项目（81090270、81090273），国家973 项目（2010CB529302），
国家科技重大专项新药创制（2009ZX09103-667，2009ZX09103-009-RC06），国家自然科学基金项目（30873005） |
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| 中文摘要: |
| 目的：证明胃癌血管靶向肽GX2 能够与胃癌血管内皮细胞特异性结合，在体内对胃癌血管具有靶向性。方法：体外实验，合
成GX2 与FITC 的复合物FITC-GX2，分离原代人脐静脉内皮细胞HUVEC,将HUVEC 与人胃癌细胞系SGC7901 共培养，建立共
培养血管内皮细胞模型来模拟胃癌血管，利用免疫荧光的方法观察GX2 与共培养内皮细胞Co-HUVEC 的结合情况；体内实验，
构建99mTc 标记的GX2 分子示踪探针，SPECT 显像观察GX2 的胃癌血管靶向性。结果：免疫荧光结果显示GX2 能与Co-HUVEC
特异性结合，而与人胃癌细胞SGC7901 不结合；SPECT 显像结果证明了GX2 在荷瘤裸鼠体内能够浓集到肿瘤部位，具有良好的
靶向性。结论：GX2 能与胃癌血管内皮细胞特异性结合，且具有在体靶向到胃癌血管的能力；GX2 具有胃癌诊断的潜在价值，并能
应用于胃癌血管抑制治疗。 |
| 英文摘要: |
| Objective: To identify targeting ability of GX2 homing to gastric cancer vasculature in vivo and in vitro. Methods: Human
umbilical vein endothelial cells (HUVEC) were isolated and cultured, and the tumor endothelial cell coculture model (Co-HUVEC)
was prepared using HUVEC and the human gastric cancer cell line SGC7901 in Transwell plates. A FITC-GX2 complex were synthesized
and used in immunofluorescence staining in vitro. Samples were examined using a fluorescence microscope and binding specificity
to Co-HUVEC was evaluated. Besides, GX2 was labeled with the radioactive isotope 99mTc, and targeting efficacy was observed using
SPECT imaging in the nude mice model bearing SGC7901 tumor xenografts. Results: Immunofluorescence staining demonstrated that
GX2 bound specifically to Co-HUVEC but not to SGC7901 cells. SPECT imaging showed that GX2 targeted the tumor tissue. Conclusions:
GX2 can bind specifically to Co-HUVEC and home to gastric cancer vasculature. GX2 can also be applied to diagnosis and therapy for
tumor angiogenesis. |
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