欢迎访问《现代生物医学进展》编辑部！

目的：研究镁补充对2 型糖尿病大鼠糖脂代谢的影响。方法：将用高脂饮食联合链脲佐菌素(STZ)方法诱发的2 型糖尿病大鼠随机分为四个组，糖尿病对照组喂饲高脂饲料，高、中、低剂量组在高脂饲料中分别加入2000、1000、200 mg/kg 的镁（以镁离子计）。自由饮食喂养四周，处死动物。用放射免疫法测血清胰岛素（Ins）水平、用葡萄糖氧化酶法测空腹血浆葡萄糖（fasting plasma glucose ，FPG），并计算胰岛素敏感指数(insulin sensitivity index，ISI)。比色法检测糖化血红蛋白（glycosylated hemoglobin,HbA1c）。用全自动生化分析仪测高密度脂蛋白胆固醇（high-density lipoprotein cholesterol ,HDLC）、甘油三酯（triglyceride ,TG）、总胆固醇（total cholesterol ,TC）。结果：高剂量组的空腹血糖、空腹血清、糖化血红蛋白、甘油三酯、总胆固醇水平均较糖尿病对照组显著性降低（P<0.05），而高密度脂蛋白胆固醇、胰岛素敏感指数较糖尿病对照组显著性升高（P<0.05）。结论：镁补充可以提高2 型糖尿病大鼠胰岛素敏感性，改善糖尿病大鼠的糖脂代谢情况。

英文摘要:

Objective: To investigate the effect of magnesium supplementation on glucose and lipid metabolism of rats with type 2 diabetes mellitus. Method: Rats with type 2 diabetes mellitus were induced with high fat diet and intraperitoneal injection with small dose of STZ, then they were divided into 4 groups randomly: high, medium, low magnesium supplementation groups and diabetes mellitus control group. Diabetes mellitus control group was feed with high-fat feed only. The dosage added in high-fat feed of the high, medium, low magnesium supplementation groups are 2000, 1000, 200 mg/kg (measured by Mg2+). All the rats ate and drunk freely. All rats were killed after four weeks. The serum insulin(Ins) level were tested with radiation immune method, fasting plasma glucose（FPG）were measured with glucose oxidase method, and calculation the insulin sensitivity index (ISI). Glycosylated hemoglobin (HbA1c) was measured by chromatometry. High-density lipoprotein cholesterol (HDLC), triglyceride (TG), total cholesterol (TC) were measured by automatic biochemical analyzer. Results: FPG, Ins, TG, TC levels of high dose group was significantly lower compared with that in the diabetic control group (P<0.05), while HDLC, ISI increased significantly compared with that in the diabetic control group (P<0.05). Conclusion: Magnesium supplement can improve insulin sensitivity, improve glucose and lipid metabolism in type 2 diabetic rats.

查看全文查看/发表评论下载PDF阅读器

关闭