| 田启飞任贵安艳新周金锋李凯王新△.胃癌特异性抗原MGb1-Ag 的表达和分布及其临床意义[J].,2012,12(13):2460-2465 |
| 胃癌特异性抗原MGb1-Ag 的表达和分布及其临床意义 |
| Expression, Distribution, and Clinical Significance of GastricCancer-Specific Antigen MGb1-Ag |
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| DOI： |
| 中文关键词: 胃癌 单克隆抗体 MGb1-Ag 免疫组织化学 分子标志物 |
| 英文关键词: Gastric cancer Monoclonal antibody MGb1 Immunohistochemistry Biomarkers |
| 基金项目:国家重点基础发展计划项目973 计划(2009CB521705)；国家自然科学基金（30973410） |
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| 中文摘要: |
| 目的：研究MGb1-Ag 在人体各系统和器官的正常组织和肿瘤中的表达和分布。观察其在胃癌中表达的临床意义。方法：（1）
免疫组织化学检测MGb1-Ag 在人体各正常组织和常见肿瘤、正常胃粘膜、慢性胃炎、肠上皮化生、不典型增生、胃癌及淋巴结转
移灶中的表达和分布，观察胃癌特异性抗原MGb1-Ag 在全身各组织器官中的分布，统计分析其在胃癌中表达与临床病理参数之
间的关系；（2）激光共聚焦检测MGb1-Ag 在胃癌细胞中的定位。结果：MGb1-Ag 仅在少数正常组织中有表达，在常见肿瘤中，主
要在胃癌、结肠癌、食管癌、胰腺癌、前列腺癌和部分肝癌、肺癌中阳性表达，而在其它肿瘤中表达较少或几乎不表达。MGb1-Ag 在
胃癌中的表达与胃癌的分化、分期和淋巴结转移密切相关。MGb1-Ag 定位于胃癌细胞系SGC-7901 的细胞膜上。结论：MGb1-Ag
是胃肠道特异性抗原，在胃癌中的表达与胃癌的分化和转移相关，是胃癌新的候选分子标志物。 |
| 英文摘要: |
| Objective: To Investigate expression and distribution of MGb1-Ag in various normal tissues and organs and malignant
tumors. To investigate the clinical value of the MGb1-Ag expression in gastric carcinomas. Methods: ①Immunohistochemical analysis
was used to examine the expression of MGb1-Ag in multiple normal or malignant tissues, normal stomach mucosa, chronic gastritis,
metaplasia, dysplasia, gastric carcinoma and lymph node metastases. Then the distribution of MGb1-Ag in multiple normal or malignant
tissues was observed, and the relationship between the expression of MGb1-Ag in gastric cancer and clinical pathology parameters was
statistically analyzed. ②The subcellular localization of MGb1-Ag in gastric cancer cells was detected by confocal laser-scanning microscopy.
Results:①MGb1-Ag expressed only in few normal tissues, but positively expressed in several common tumors, such as gastric
cancer, colonic cancer, esophageal carcinoma, pancreatic cancer, prostate cancer and some hepatic carcinoma, lung cancer. And
MGb1-Ag was negative in all other detected tumors. In addition, the expression of MGb1-Ag correlated with differentiation, stages and
lymph node metastasis of gastric carcinoma. ② The subcellular localization of MGb1-Ag in gastric cancer cell SGC7901 was mainly in
cytomembrane. Conclusion: The expression of gastrointestinal-specific antigen MGb1-Ag correlated with differentiation and metastasis
of gastric cancer, so MGb1-Ag could be used as a novel bio-marker candidate. |
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