文章摘要
刘海珍林琍宗文霞.内皮型一氧化氮合酶基因多态性与高血压合并脑 梗塞的关系[J].,2012,12(12):2334-2336
内皮型一氧化氮合酶基因多态性与高血压合并脑 梗塞的关系
Relationship between 894G/T Polymorphism of Endothelial Nitric OxideSynthase Gene and Essential Hypertension Complicated with CerebralInfarction
  
DOI:
中文关键词: 内皮型一氧化氮合酶  基因多态性  原发性高血压  脑梗塞
英文关键词: Endothelial nitric oxide synthase  Polymorphism  Essential hypertension  Cerebral infarction
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作者单位
刘海珍林琍宗文霞 武汉大学附属湖北省中山医院 
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中文摘要:
      目的:探讨内皮型一氧化氮合酶(eNOS)基因894G/T 多态性与原发性高血压(EH)合并脑梗塞(CI)的关系。方法:应用聚合 酶链反应限制性片段长度多态性方法检测湖北地区汉族74 例健康者(NT 组)、103 例原发性高血压无合并症者(EH 组)及70 例 原发性高血压合并脑梗塞者(EH-CI 组)的eNOS 基因型;生化技术测定其血脂、一氧化氮代谢物(NOM)水平。结果:EH 组及 EH-CI 组患者的T 等位基因频率分别为0.224 和0.321,均显著高于NT 组(P<0.05);且两者之间的T 等位基因频率差异显著性 (P<0.05);EH-CI 组中,GT+TT 基因型者的舒张压显著高于GG 基因型者(P<0.05),而NOM 显著低于GG 基因型者。结论: eNOS 基因894 位G/T 多态性可能与汉族高血压病患者伴脑梗塞有关,该位点多态性可能使T 等位基因携带者NOM 减少,进而 参与EH-CI 发病。
英文摘要:
      Objective: To investigate the relationship between 894 G/T polymorphism of endothelial nitric oxide synthase (eNOS) gene and essential hypertension complicated with cerebral infarction in Chinese population. Methods: With polymerasechain reaction restriction fragment lenth polymorphism(PCR-RFLP), eNOS genotyping was performed in 74 unrelated healthy controls(NT group)and 103 hypertensive patients without cardio-cerebrovascular diseases (EH group) and 70 hypertensive patients with cerebral infarction(EH-CI group),whose plasma lipid levels and nitric oxide metabolites (NOM) were measured. Results: The frequencies of T allele of eNOS gene in two groups of hypertention were significantly higher than that in NT group respectively (P<0.05) ,and the frequencies of T allele of eNOS gene in EH-CI group were higher(P<0.05). Diastolic blood pressure (DBP) for eNOS GT+TT genotype was significantly higher in EH-CI group than that for GG genotype in EH group. (P<0.05) . The level of NOM was lower in GT+TT genotypes than that in GG genotype(P<0.05).Conclusion: eNOS gene G894T polymorphism could contribute to the development of essential hypertension, and to be the incidence of CI in the hypertensives. T allele might be involved in the aetiology of EH-CI by reducing NO release of endothelium.
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