| 孟德友汲晨峰季宇彬.不同硫取代度昆布多糖硫酸酯的合成及理化性质初步研究[J].,2012,12(12):2298-2301 |
| 不同硫取代度昆布多糖硫酸酯的合成及理化性质初步研究 |
| Synthesis of Different Degree of Substitution of LAMS and PreliminaryStudy on Physical and Chemical Properties |
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| DOI： |
| 中文关键词: 昆布多糖硫酸酯 取代度 多糖含量 分子量 |
| 英文关键词: LAMS Degree of substitution Polysaccharide content Molecular weight |
| 基金项目:黑龙江省自然科学基金项目(QC2011C100)，黑龙江教育厅科学技术研究面上项目(12511136) |
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| 中文摘要: |
| 目的：对昆布多糖进行不同硫取代度的硫酸酯化修饰，并对其产物的硫酸基含量、糖含量与分子量进行检测，为研究不同硫
取代度昆布多糖硫酸酯的生物活性奠定物质基础。方法：采用氯磺酸- 吡啶法对昆布多糖进行硫酸化修饰，通过改变硫酸化修饰
条件，来制取不同硫酸基取代度的昆布多糖硫酸酯；利用盐酸水解- 硫酸钡比浊法测定昆布多糖硫酸酯的硫酸基含量，并通过公
式求得其硫取代度；用苯酚- 硫酸法测定昆布多糖硫酸酯的多糖含量，并使用HPGPC 法测定其分子量。结果：两种不同硫取代度
昆布多糖硫酸酯的硫酸基含量分别为37.8%、45.92%，取代度分别为1.07、1.51，糖含量分别为44.52%、37.19%，分子量分别为
13000、16000。结论：利用氯磺酸- 吡啶法对昆布多糖进行硫酸酯化修饰，该方法可以获取不同取代度产物，酯化率高。 |
| 英文摘要: |
| Objective: To sulfate the laminarin for different degree of substitution. And its sulfate content, sugar content and
molecular testing for the study of different degree of substitution of LAMS lay the material foundation of biological activity. Methods:
Using chlorosulfonic acid - pyridine method for sulfation of laminarin, sulfated by changing conditions, preparation of different degree of
substitution of sulfate laminarin sulfate; the use of barium sulfate turbidimetric method LAMS of sulfate content, and calculate the degree
of substitution; phenol - sulfuric acid method for the determination of laminarin sulfate polysaccharide content and its molecular weight
determined using HPGPC. Results: LAMS of two different sulfur degree of substitution of sulfate contents were 37.8%, 45.92%, the
degree of substitution were 1.07,1.51, sugar contents were 44.52%, 37.19%, and molecular weight were 13000, 16000. Conclusion：
LAMS was prepared from laminarin by chlorsulfonic acid-pyridine method. The rate of esterification are high and preparation of a
different degree of substitution of the product by this method. |
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