| 唐桦徐晓芃冯浩肖伟荣.白藜芦醇对紫外线照射后人角质形成细胞AQP3 表达的影响[J].,2012,12(12):2275-2277 |
| 白藜芦醇对紫外线照射后人角质形成细胞AQP3 表达的影响 |
| Effect of Resveratrol on Expression of Aquaporin 3 in Normal HumanEpidermal Keratinocytes Irradiated by UVB |
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| DOI： |
| 中文关键词: 白藜芦醇 水通道蛋白3 角质形成细胞 中波紫外线 |
| 英文关键词: Resvetatrol Aquaporin 3(AQP3) Normal human epidermal keratinocytes（NHEK） Ultraviolet B(UVB) |
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| 中文摘要: |
| 目的：探讨白藜芦醇对紫外线照射后人皮肤角质形成细胞水通道蛋白3（AQP3）表达的影响及意义。方法：原代培养人皮肤
角质形成细胞，采用UVB（20mJ/cm2，40mJ/cm2）照射角质形成细胞后，立即加入0.1mmol/L 的白藜芦醇进行干预。RT-PCR 检测
照射前后角质形成细胞中AQP3 mRNA 的表达量，并用羟胺法、比色法、TBA 法检测照射前后细胞超氧化物歧化酶（SOD）、谷胱
甘肽过氧化物酶（GSH-Px）活性及丙二醛（MDA）含量。结果：1.UVB 照射后角质形成细胞AQP3 mRNA 的表达量下降（P<0.05），
且UVB 照射剂量越大，AQP3 mRNA 下降越显著（P<0.05）。2.白藜芦醇能显著增加UVB 照射后角质形成细胞SOD 和GSH-Px
活性，并降低细胞MDA 含量（P<0.05）。3.白藜芦醇能显著抑制UVB 导致的角质形成细胞AQP3 mRNA 下降（P<0.05）。结论：白藜
芦醇可能通过抑制UVB 导致的AQP3 mRNA 下降，及提高氧化酶活性、清除自由基的功能，从而延缓皮肤衰老。 |
| 英文摘要: |
| Objective: To investigate the effect of resveratrol on expression of AQP3 in normal human epidermal keratinocytes
(NHEK) irradiated by UVB, and discuss its function in the process of skin aging. Methods: Preparation of skin keratinocyte primary
cultures. NHEK irradiated by UVB (20mJ/cm2，40mJ/cm2) were immediately treated with 0.1mmol/L resvetatrol, then observe the change
of the expression of AQP3 mRNA by RT-PCR, and the change of SOD, GSH-Px and MDA by Hydroxyl amine method, colorimetric method
and TBA method. Results: 1. The expression level of AQP3 mRNA after UVB irradiation was significantly lower than its level before
UVB irradiation (P<0.05). The more the UVB exposure dose it received, the greater the expression level of AQP3 mRNA decreased.
2. Resvetatrol increased the activity of SOD and GSH-Px, decreased MDA's content of NHEK irradiated by UVB (P<0.05). 3. Resvetatrol
inhibited the decrease of AQP3 mRNA irradiated by UVB (P<0.05). Conclusion: Resvetatrol can attenuate UVB-induced down-regulation
of AQP3, increase oxydase's activity and clean the free radical, and accordingly to delay skin aging. |
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