| 王铸何霞冯发深关琳琳徐霖张定梅汪杨罗燕芬曹开源△.Endophilin B1 基因及蛋白的生物信息学分析[J].,2012,12(12):2212-2219 |
| Endophilin B1 基因及蛋白的生物信息学分析 |
| Bioinformatics Analysis of Endophilin B1 Gene and Protein |
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| DOI： |
| 中文关键词: Bif-1/Endophilin B1 BAR domain SH3 domain 生物信息学 |
| 英文关键词: Bif-1/Endophilin B1 BAR domain SH3 domain Bioinformatics |
| 基金项目:国家自然科学基金资助项目（81071760） |
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| 中文摘要: |
| 目的：利用生物信息学方法分析人Endophilin B1 基因以及蛋白的结构，为进一步研究其功能和参与的调控机制提供一定的
理论依据。方法：通过GenBank 搜索Endophilin B1 基因及蛋白序列，采用生物信息学方法分析该基因在不同物种中的差异，分析
该蛋白的亚细胞定位，二级结构，功能域以及抗原表位。结果：该基因编码一个长度为365 个氨基酸的蛋白，具有两个BAR 和
SH3 两个功能域。Endophilin B1 蛋白理论分子量为40796.3，理论等电点为5.78。二级结构中α 螺旋（H）占56.44%,β 折叠(E)占
5.48%, 无规卷曲占38.08%。Endophilin B1 蛋白含有4 个可能的N 连接糖基化位点，5 个潜在的酪蛋白激酶II 磷酸化位点，7 个豆
蔻酰基化位点，3 个PKC 磷酸化位点以及2 个酪氨酸激酶磷酸化位点。并进一步利用DNAstar 软件分析了了该蛋白的抗原表位。
结论：利用生物信息学预测出的结构和功能信息，能为Endophin B1 蛋白的相关研究提供一定的信息基础。 |
| 英文摘要: |
| Objective: To investigate the function and regulatory mechanisms of human Endophilin B1 gene and protein in human
disease. Methods: Search Endophilin B1 gene and protein sequences from GenBank. The differences in different species, subcelluar
location, secondary struture, functional domains and epitopes were analyzed by using Bioinformatics tools. Results: The gene encodes a
length of 365 amina acid protein with two functional domains, BAR and SH3 domain. Its molecular weight is 40796.3, theoretical
isoelectric point is 5.78. α-helix secondary structure (H) accounted for 56.44%, β fold (E) accounted for 5.48%, random coil accounting
for 38.08%. Endophilin B1 protein contains four potential N-linked glycosylation sites, five potential casein kinase II phosphorylation
sites, seven cardamom acylation sites, three PKC phosphorylation sites and two tyrosine kinase phosphorylation sites. And a further
analyzed of its protein epitopes by DNAstar software was completed. Conclusion: More biological information about Endophilin B1 gene
and protein was analyzed, which provide some basic information for further research. |
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