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目的：评价HA 纳米载体转hGM-CSF 基因的HepG2 疫苗的抗肿瘤活性。方法：SCID 小鼠20 只腹腔内注射健康志愿者外周血淋巴细胞（1×107/ ml）1.0 ml，同时背部皮下接种人肝癌HepG2 细胞(1×107ml)0.2 ml。当皮下移植瘤体积长至100 mm3 时，随机分四组：Ⅰ组，60Co 照射的转染GM-CSF 基因的HepG2 细胞组，Ⅱ组，60Co 照射的HepG2 细胞组，Ⅲ组，生理盐水组，Ⅳ组，接种前，每组5 只。MTT 法检测各组小鼠脾细胞CTL 活性，ELISA 法检测血清IL-4 和IL-12 的水平。结果：转染GM-CSF 基因的 HepG2 疫苗组小鼠脾细胞CTL 活性明显高于其余组（P<0.05）；同时血清Th1 类细胞因子IL-12 的水平明显升高（P<0.05），而Th2 类细胞因子IL-4 的水平无统计学意义（P>0.05）。结论：HA 纳米载体转hGM-CSF 基因的HepG2 疫苗可刺激机体产生特异性免疫反应。

英文摘要:

Objective: To investigate the anti-tumor effects and mechanism of HepG2 tumor vaccine transfected hGM-CSF gene mediated by HA nanoparticles. Methods: Health human peripheral blood lymphocytes were isolated from peripheral blood of healthy donors and intraperitoneal injected into SCID mice at 2×107/ ml. Simultaneously, hepatocellular carcinoma cells were intracutaneously injected into SCID mouse at 2×107/ ml. When the xenografted to a 100 mm3, mice was randomly divided into 4 groups: Group 1,HepG2 cells transfected with GM-CSF Gene after 60Co irradiation group; Group 2, HepG2 cells after 60Co irradiation group; Group 3, normal saline group: Group 4, uninjected group. Every group had 5 mice. Cytotoxic rates were detected by using MTT assay. The level of IL-12 and IL-4 in serum was detected by ELISA method. Results: The activaty of CTL were significantly higher in group HepG2 tumor vaccine transfected hGM-CSF gene mediated by HA nanoparticles than those in other groups （P<0.05）; Serum level of Th1-type cytokine IL-12 increased after vaccination（P<0.05）, whereas Th2-type cytokine IL-4 showed no significant changes（P>0.05）. Conclusion: The HepG2 tumor vaccine transfected hGM-CSF gene mediated by HA nanoparticles can induce immune response.

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