| 谌海燕1 侯丽1 李冬云1 柴立民2 陈信义1，2.P-gp 和MDR1 在三种乳腺癌细胞中表达差异研究[J].,2012,12(10):1808-1811 |
| P-gp 和MDR1 在三种乳腺癌细胞中表达差异研究 |
| The Study of Differential Expression of P-gp and MDR1 in Three BreastCancer Cells |
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| DOI： |
| 中文关键词: 乳腺癌 耐药 P-gp MDR1 |
| 英文关键词: Breast cancer Resistant P-gp MDR1 |
| 基金项目:北京中医药大学自主创新课题(2009TYBZZ-XS057) |
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| 中文摘要: |
| 目的：比较P-gp 和MDR1 在人乳腺癌敏感细胞（MCF-7/S）和耐药细胞（MCF-7/ADR、MCF-7/TAM）中的表达差异，初步探讨
乳腺癌细胞对阿霉素与对三苯氧胺产生耐药机制的区别。方法：采用免疫细胞化学法、流式细胞术检测P-gp，采用实时荧光定量
PCR 法检测MDR1 在三种乳腺癌细胞中的表达情况。结果：在MCF-7/ADR 细胞中P-gp 和MDR1 均呈高表达，阳性表达率与
MCF-7/S 细胞比较，有统计学意义（P＜0.01）。在MCF-7/TAM 细胞中P-gp、MDR1 均呈低表达，与MCF-7/S 细胞比较，无统计学意
义（P＞0.05）。结论：P-gp 和MDR1 的高表达是乳腺癌细胞对阿霉素产生耐药的主要机制，而并非是乳腺癌细胞对三苯氧胺产生耐
药的机制。 |
| 英文摘要: |
| Objective: To compare the differential expression of P-gp and MDR1 in human breast cancer sensitive cells（MCF-7/S）
and resistant cells （MCF-7/ADR, MCF-7/TAM）, and to preliminary distinguish the mechanism between breast cancer cells resistant to
adriamycin and to tamoxifen. Methods: We detected the expression of P-gp by using the methods of immunocytochemistry and flow
cytometry, and detected the expression of MDR1 by using real-time fluorescence quantitative PCR in the three breast cancer cells.
Results: In MCF-7/ADR cells, P-gp and MDR1 showed a high expression, which had a statistical significance compared with MCF-7/S
cells (P<0.01). In MCF-7/TAM cells, P-gp and MDR1 showed a low expression, which had no statistical significance compared with
MCF-7/S cells (P>0.05). Conclusion: The high expression of P-gp and MDR1 is the main mechanism in breast cancer cells resistant to
adriamycin, and is not the mechanism in breast cancer cells resistant to tamoxifen. |
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