| 李双李武平刘冰孙惠英宋向阳闫沛白艳玲王蕾.西安市MRSA 耐消毒剂基因qacA/B 的检测[J].,2012,12(9):1716-1718 |
| 西安市MRSA 耐消毒剂基因qacA/B 的检测 |
| A Investigation of Disinfectant-Resisitant Gene QacA/B inMeticillin-Resisitant Staphylococcus Aureus Isolated from Xi'an's Hospital |
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| DOI： |
| 中文关键词: 耐甲氧西林金黄色葡萄球菌 消毒剂 抗性 |
| 英文关键词: Meticillin-resisitant Staphylococcus aureus Disinfectants Resistance |
| 基金项目:陕西省科学技术研究发展计划项目（2110k16-01-07） |
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| 中文摘要: |
| 目的：了解西安市三甲综合医院耐甲氧西林金黄色葡萄球菌（MRSA）qacA/B 基因的携带情况，以便掌握其对消毒剂的耐药
情况。方法：收集临床分离的152 株耐甲氧西林金黄色葡萄球菌，采用聚合酶链反应检测qacA/B 基因,并将qacA/B 基因扩增产
物进行测序，测序结果进行比对。结果：152 株耐甲氧西林金黄色葡萄球菌中有11 株检出qacA/B 阳性，qacA/B 携带率7.2%；测序
结果比对发现，4 株qacA 的基因编码序列同一位点均有一个碱基发生突变（C→T），苏氨酸突变为异亮氨酸。结论：该地区临床分
离的耐甲氧西林金黄色葡萄球菌中qacA/B 携带率相对较低，但也存在抵抗消毒剂的风险，临床应加强消毒剂使用的管理，阻止
MRSA 的传播，减少感染的发生。 |
| 英文摘要: |
| Objective: To investigate the disinfectant-resistant gene qacA/B in meticillin-resisitant Staphylococcus aureus isolated
from Xi'an's hospital, in order to know the aureus 's sensitivity of disinfectant. Methods: A total of 152 MRSA strains were isolated.
Polymerase chain reaction (PCR) was implied to analyze the prevalence of gene qacA/B in these stains. Last, taking the positive-qacA/B
gene's output into testing the DNA sequence and analyzing. Results: Of the 152 MRSA strains, 11 were positive for qacA/B gene. The
prevalence of gene qacA/B in these stains was 7.2%. One nucleotide take place missense mutation （C→T）in some site at the coding
section of qacA/B gene sequence of 4 strains. Conclusions: There were not very high positive percentages of relevant resistant gene
qacA/B in MRSA isolated from clinical, but we also take seriously on the phenomenon of disinfectant resistance and make good use of
disinfectants to prevent MRSA transferring, even more to decrease the prevalence of infection. |
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