| 於进文蔡越王忠超白云刚刘焕暴军香马进△.[J].,2012,12(9):1632-1635 |
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| DOI： |
| 中文关键词: 模拟失重 胸主动脉 氧化应激 大鼠 |
| 英文关键词: Simulated microgravity Thoracic aorta Oxidative stress Rat |
| 基金项目:国家自然科学基金项目（30971423） |
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| 中文摘要: |
| 目的：观察模拟失重对大鼠胸主动脉氧化应激水平的影响，探讨其可能机制。方法：采用3 周尾部悬吊大鼠模型模拟失重状
态，通过DHE 荧光探针技术观察大鼠动脉血管超氧阴离子水平变化，通过比色法测定大鼠动脉血管丙二醛（MDA）含量，通过蛋
白印记技术观察悬吊（SUS）大鼠和正常对照（CON）大鼠动脉血管NOX4、p22phox 的表达变化。结果：尾部悬吊3 周后，SUS 组大
鼠胸主动脉超氧阴离子水平较CON 组明显增高,SUS 组（0.849±0.023 nmol/mg protein）大鼠MDA 含量较CON 组（0.575±0.054
nmol/mg protein）明显增加；SUS 组大鼠胸主动脉的p22phox 及NOX4 蛋白表达均较CON 组明显增强。结论：模拟失重3 周可使
大鼠胸主动脉氧化应激水平明显增高，p22phox 及NOX4 蛋白表达明显增多，结果提示，尾部悬吊模拟失重状态下氧化应激水平
增高可能与NADPH 氧化酶表达增高有关。 |
| 英文摘要: |
| Objective: To investigate the level of oxidative stress in tail suspended rats and the mechanism. Methods: 3-week tail
suspended rats were adopted as the animal model of simulated microgravity. DHE fluorescent probe technique was applied to evaluate
the level of superoxide anion. Colorimetry was taken to detect the MDA content. Western blot analysis was taken to compare the expression
of NOX4 and p22phox between the SUS and CON groups. Results: Compared with CON, the level of superoxide anion enhanced
significantly in SUS rats, while the content of MDA increased in SUS rats. The expression of both p22phox and NOX4 increased in SUS
rats as a result of 3-week simulated microgravity. Conclusion: The level of oxidative stress in rats increased by 3-week simulated microgravity,
and increased ROS level in tail suspended rats might associated with the enhanced expression of NADPH oxidase. |
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