| 郑志刚吴楠周倜刘栋陈勇.内质网应激预处理对人正常肝细胞缺氧再复氧损伤的保护作用[J].,2012,12(8):1426-1429 |
| 内质网应激预处理对人正常肝细胞缺氧再复氧损伤的保护作用 |
| Protective Effect of Endoplasmic Reticulum Stress Preconditioningon the HL-7702 Cell Injuried by Hypoxia-Reoxygenation |
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| DOI： |
| 中文关键词: 内质网应激 预处理 人正常肝细胞 缺氧再复氧损伤 |
| 英文关键词: Endoplasmic reticulum stress Preconditioning HL7702 cell Hypoxia/ reoxygenation injury |
| 基金项目:国家自然科学基金（81070363） |
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| 中文摘要: |
| 目的：研究内质网应激预处理对人肝细胞缺氧复氧损伤的保护作用。方法：将培养的人肝细胞分为4 组：正常对照（C）组、细
胞缺氧复氧损伤（H/R）组、内质网应激（ER）组、内质网应激预处理（ERP+ H/R）组。收集各组细胞，以流式细胞仪检测细胞凋亡，
Western-bloting 及RT-PCR 检测内质网应激特异蛋白GRP78 表达水平，并通过透射电镜观察各组细胞超微结构改变。结果：
ERP+ H/R 组细胞凋亡率明显低于H/R 组（P<0.05），ER 及ERP+ H/R 组GRP78 蛋白表达明显高于H/R 组（P<0.05）。结论：内质网
应激预处理对肝细胞缺氧复氧损伤具有明显的保护作用，内质网应激特异性蛋白GRP78 可能在肝细胞缺氧复氧损伤中作为一种
关键性的保护蛋白出现。 |
| 英文摘要: |
| Objective: To study the protective effects on the endoplasmic reticulum stress preconditioning on human hepatic cell
hypoxia-reoxygenation injury. Methods: Cultured human liver cells into four groups, namely normal control (C) group, hypoxia-reoxygenation
(H / R) group, endoplasmic reticulum stress (ER) group, endoplasmic reticulum stress preconditioning(ERP + H / R) group. The
cells were collected to detect apoptosis by flow cytometry, Western-bloting and RT-PCR detection of endoplasmic reticulum stress
protein GRP78-specific expression, and ultrastructural changes of cells were observed by transmission electron microscopy. Results: ERP
+ H / R cell apoptosis was significantly lower than H / R group (P <0.05), ER and ERP + H / R group GRP78 protein expression was
significantly higher than the H / R group (P <0.05). Conclusion: Endoplasmic reticulum stress preconditioning on hypoxia-reoxygenation
injury of liver cells has a significant protective effect. Endoplasmic reticulum stress-specific protein GRP78 is a key protective protein in
liver cells injured by hypoxia-reoxygenation. |
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