陈丹于壮△ 潘华政杨堃刘相萍.RalA RhoC 在非小细胞肺癌组织中的表达及意义[J].,2012,12(7):1296-1299 |
RalA RhoC 在非小细胞肺癌组织中的表达及意义 |
The Expression and Significance of RhoC and RalAin Non-Small Cell Lung Carcinoma |
|
DOI: |
中文关键词: 非小细胞肺癌 RhoC RalA 荧光定量PCR |
英文关键词: Lung carcinoma RhoC RalA Real-Time PCR |
基金项目:青岛市科技计划项目(06-2-2-5-nsh-1);山东省科技发展计划项目(2010GWZ20206) |
|
摘要点击次数: 666 |
全文下载次数: 874 |
中文摘要: |
目的:观察RalA 和RhoC 基因在非小细胞肺癌组织细胞中的表达变化,探讨它们的表达在非小细胞肺癌发展和侵袭转移中
的作用。方法:通过实时荧光定量PCR(FQ-PCR)检测RalA、RhoC 在60 例非小细胞肺癌患者的肿瘤组织和癌旁组织细胞中的表
达,了解其与非小细胞肺癌临床病理学指标的关系。结果:RhoC 在非小细胞肺癌组织细胞中的表达较癌旁组织显著增高(P<0.
05), 且与淋巴结的转移、TNM 分期和分化程度密切相关(P<0.05)。而RalA 在非小细胞肺癌组织细胞中的表达量低于癌旁组织
(P<0.05),与淋巴结转移无明显相关(P>0.05)。二者在鳞、腺癌中表达均无差异(P<0.05)。结论:肺癌组织细胞中RhoC 表达升高
及RalA 表达异常下调与非小细胞肺癌的发生、发展及侵袭机制关系密切。 |
英文摘要: |
Objective: To investigate the mRNA express of Ral A and RhoC in lung carcinoma and their clinical significance.
Methods: The quantity of RhoC and RalA were detected by TaqMan probe based fluorescence quantitative polymerase chain reaction
(FQ-PCR) in 60 cases of lung carcinoma,corresponding para-cancer tissues. Results: In lung carcinoma,the mean quanitities of RhoC
mRNA were higher than that in corresponding para-cancer tissues (P<0.05),and was closely related with lymph node metastases;TNM
stage and histological grade. The mean quanitities of RalA mRNA was higher in corresponding para-cancer tissues (p<0.05),and no
significant association was found bettwen RalA and lymph node metastases(P>0.05).In lung caner,both RalA and RhoC mRNA have no
difference between squamous cell carcinoma and adenocarcinoma. Conclusion: Preserved expressin of Rho C and defective expressin of
RalA can be concerned with NSCLC. |
查看全文
查看/发表评论 下载PDF阅读器 |
关闭 |