| 蔡宏凤1 王慎旭1 卢媛媛1 王福2 张荣庆1 曹丰1△.bFGF 诱导肺腺癌细胞系A549 上皮间质转化[J].,2012,12(7):1241-1243 |
| bFGF 诱导肺腺癌细胞系A549 上皮间质转化 |
| bFGF Induced Epithelial-Mesenchymal Transition of Human LungAdenocarcinoma Cell Line A549 |
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| DOI： |
| 中文关键词: 碱性成纤维细胞生长因子bFGF A549 细胞 上皮间质转化 肺癌 |
| 英文关键词: Basic fibroblast growth factor A549 cell Epithelial-mesenchymal transition Lung cancer |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨上皮间质转化（epithelial-mesenchymal transition，EMT）过程在肺癌侵袭转移中的作用。方法：体外培养A549 细
胞，以bFGF（10ng／ml）进行干预后，倒置相差显微镜观察细胞形态学变化；间接免疫荧光观察上皮细胞标志物E-cadherin 和间质
细胞标志物vimentin 蛋白表达的变化；采用细胞划痕试验检测bFGF 对A549 细胞迁移能力的影响；采用transwell 小室试验检测
bFGF 对A549 细胞侵袭能力的影响。结果：bFGF（10ng／ml）干预后，在倒置相差显微镜下观察，A549 细胞形态变成了梭形，形态
如同成纤维细胞。间接免疫荧光显示A549 细胞E-cadherin 表达随时间延长逐渐减弱，而vimentin 表达逐渐增强。细胞划痕试验
显示，bFGF 干预后细胞迁移能力提高。Transwell 小室试验显示，bFGF 干预后细胞侵袭能力提高。结论：bFGF 在体外诱导肺腺癌
细胞系A549 细胞发生上皮间质转化，上皮间质转化是肺癌侵袭转移的重要机制之一。 |
| 英文摘要: |
| Objective: To investigate the effect of epithelial to mesenchymal transition (EMT) on the invasion and metastasis of
lung cancer. Methods: A549 cells cultured in vitro were treated by bFGF (10ng/ml), then the morphological changes were detected by
inverted phase contrast microscopy; The expression of the epithelial marker E-cadherin and mesenchymal marker vimentin was detected
by Indirect immunofluorescence; The effect of bFGF on migration of A549 was detected by scarification assay; The effect of bFGF on
invasion of A549 was detected by transwell assay. Results: After treated by bFGF (10ng/ml), A549 cells turn from pebble shape to
fusiform shape, a myofibroblast-like morphology. The expression of E-cadherin decreased, and the expression of vimentin increased.
Scarification test showed that the migration potential of A549 cells was significantly increased after bFGF treatment. The invasive
capacity of A549 increased after bFGF treatment. Conclusion: bFGF may contribute to EMT in lung adenocarcinoma cell line A549.
EMT is an important mechanism for invasion and metastasis of lung cancer. |
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