| 杨帆陈仙△ 丁军刘莉莎唐亚纯.肾盂灌注冲洗并发全身炎症反应综合征猪模型的建立[J].,2012,12(6):1054-1058 |
| 肾盂灌注冲洗并发全身炎症反应综合征猪模型的建立 |
| Intrapelvic Perfusion of Systemic Inflammatory Response Syndromein Pig models |
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| DOI： |
| 中文关键词: 经皮肾镜取石术 全身炎症反应综合征 猪 模型 |
| 英文关键词: Percutaneous nephrostolithotomy Systemic inflammatory response syndrome Pig Model |
| 基金项目: |
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| 摘要点击次数: 905 |
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| 中文摘要: |
| 目的：建立一种肾镜下肾盂灌注冲洗并发全身炎症反应综合征（SIRS）的猪模型。方法：家猪10 头，随机分为假手术组和模
型组，每组按时间点分为基础状态(Basic)、术后0h、术后6h、术后12h 和术后24h 5 个亚组。猪肾穿刺造瘘，利用自体盲肠内容物
加入肾盂冲洗液中，模拟经皮肾镜取石术(PCNL)持续肾盂灌注冲洗1h。每个时间点观测肛温(T)、呼吸(RR)、心率(HR)；采血行血
细胞计数分析(WBC、RBC、PLT)、肾功能(Cr、BUN、β2-MG）测定、血清IL-6、IL-10、TNF-α 含量测定；用光镜和电镜观察肾脏组织
病理变化。结果：模型组在术后6 h-24 h 各项指标与假手术组相比变化明显，差异有统计学意义(P<0.05 或<0.01)。模型组肾脏组
织光镜和电镜下观察病理切片均较假手术组有明显的形态学改变。结论：本模型较好地模拟了临床病人PCNL 并发SIRS 的病理
生理过程，为进一步研究其发病机制和预防治疗具有重要意义。 |
| 英文摘要: |
| Objective: To establish a model of intrapelvic perfusion of systemic inflammatory response syndrome (SIRS) in pigs.
Methods: Ten pigs were randomly divided into sham group and model groups. Each group was further divided into five subgroups according
to the time of preoperative(Basic), postoperative 0 h, 6 h, 12 h and 24 h. The pigs was made a nephrostomy, and perfused the kidney with
the perfusion fluid which was added to caecum content of pigs 1 hour. Rectal temperature(T), respiratory rate (RR), heart rate (HR); Peripheral
blood cell counts(WBC, RBC, PLT), renal function test(Cr, BUN, β2-MG), and the serum levels of IL-6, IL-10 and TNF-α were record
and analyzed; the pathological changes of kidney tissue were observed by optical microscope and electron microscopic. Results: Compared
with that in the sham-operation group, the model-group each target change was obvious in postoperative 6h to 24 h(P <0.05 or <0.01). Pathomorphology
showed there was change in acute inflammatory. Conclusions: The model could simulate the pathophysiologic process of
PCNL complicated with SIRS. It is important for us to further study the pathogenesis, prophylaxis and treatment of SIRS. |
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