| 秦明1 杨琦1 王景杰1 饶志仁2 赵曙光1 黄裕新1△ 叶文广1 姚庆林1.电针足三里穴对糖尿病胃轻瘫大鼠延髓多巴胺能神经元和
星形胶质细胞活性的影响[J].,2012,12(6):1006-1008 |
| 电针足三里穴对糖尿病胃轻瘫大鼠延髓多巴胺能神经元和
星形胶质细胞活性的影响 |
| Effects of Electroacupuncture on Medullary Dopaminergic Neurons andAstrocytes Activation in Diabetic Gastroparesis Model Rats |
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| DOI： |
| 中文关键词: 电针 胃轻瘫 胃电 延髓 神经元 星形胶质细胞 |
| 英文关键词: Electroacupuncture Dopaminergic neurons Astrocytes Gastroparesis |
| 基金项目:国家自然科学基金资助项目（30801487）；陕西省中医药管理局中医药科研课题基金资助项目(36) |
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| 中文摘要: |
| 目的：研究电针足三里穴对糖尿病胃轻瘫大鼠延髓多巴胺能神经元内酪氨酸羟化酶（tyrosine hydroxylase , TH）和星形胶质
细胞内胶质原纤维酸性蛋白(Glial Fibrillary Acidic Protein, GFAP)表达的影响。方法：32 只实验大鼠分为空白对照（空白）组、糖尿
病胃轻瘫模型（模型）组、模型组+ 电针足三里穴（足三里）组和模型组+ 电针非经非穴（非经非穴）组（每组8 只）。模型制备采用
腹腔注射5 %四氧嘧啶和熟地灌胃诱导的方法。实验3 周后取大鼠延髓进行抗TH 和抗GFAP 的单一和双重免疫组化染色，观察
并记数TH 和GFAP 在延髓内的表达。结果：与空白组比较，各实验组TH 阳性多巴胺能神经元和GFAP 阳性星形胶质细胞集中
表达于延髓迷走孤束复合体内，有明显的定位特点；高倍镜下观察到TH 阳性神经元周围有大量GFAP 阳性星形胶质细胞包绕。
各组TH 和GFAP 表达以模型组最高；而足三里组TH 阳性多巴胺能神经元数量明显减少（31.3±4.4→16.8±3.2），GFAP 阳性产
物表达明显降低（113.8±7.6→95.4±8.4），且它们之间有统计学意义（P<0.01）；非经非穴组与模型组之间差异没有统计学意义。结
论：针刺调节糖尿病胃运动功能障碍大鼠与其调控延髓多巴胺能神经元及其周围的星形胶质细胞功能活动有关。 |
| 英文摘要: |
| Objective: To investigate the neural mechanisms of electroacupuncture(EA) effects on the diabetic gastoparesis (DGP),
and to investigate the expression of tyrosine hydroxylase (TH) in dopaminergic neurons and glial fibrillary acidic protein (GFAP) in
astrocytes in bulbus of diabetic rats. Methods: 32 Rats were divided into 4 groups: control group, DGP model group, DGP plus EA
Zusanli acupoint group and DGP plus EA non-acupoint group. DGP model rats were induced by intraperitoneal injection (i.p.)5% alloxan
plus poured libosch. After 3 weeks, the expression of TH and GFAP immunoreactivity in bulbus was investigated and calculated by using
single or double immunohistochemical method. Results: In the experimental groups, the TH-positive dopaminergic neurons and GFAPpositive
astrocytes expressed mainly in the vagal-solitary complex (VSC) of bulbus. Under the high microscope, those dopaminergic
neurons were surrounded with their neighboring astrocytes. In the DGP group and EA non-acupoint group, the expression of TH and
GFAP immunoreactivity were higher than those in the EA Zusanli groups (P<0.01). Conclusion: EA can improve the gastric motility in
DGP model rats. This function may be connected with its regulating the TH-positive dopaminergic neurons and GFAP-positive astrocytes
activation in VSC. |
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