| 经纬胡先贵△ 周旭宇金钢邵成浩.骨桥蛋白在胰腺癌中的表达及其临床意义[J].,2012,12(5):915-917 |
| 骨桥蛋白在胰腺癌中的表达及其临床意义 |
| Expression of Osteopontin in Pancreatic Cancer and ItsClinical Significance |
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| DOI： |
| 中文关键词: 胰腺癌 骨桥蛋白 RT-PCR 免疫组织化学 |
| 英文关键词: Pancreatic cancer Osteopontin RT-PCR Immunohistochemistry |
| 基金项目: |
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| 中文摘要: |
| 目的：研究骨桥蛋白OPN 在胰腺癌中的表达水平及其与临床病理特征的关系。方法：采用RT-PCR 和免疫组织化学方法
分别检测50 例胰腺癌手术切除标本和20 例癌旁正常胰腺组织中OPNmRNA 及其蛋白水平的表达。结果：胰腺癌组织中OPN
mRNA 高表达率为90%（45/50）明显高于其在癌旁正常胰腺组织中的表达15 %（3/20），差异有统计学意义( P<0.01)；OPN 蛋白的
阳性率为86.0 %（43/50），明显高于癌旁正常胰腺组织中的表达30%（6/20），差异有统计学意义( P<0.01)；OPN 在胰腺癌组织中表
达与其淋巴结转移明显相关（P<0.05）。结论：OPN 在胰腺癌中的过表达对胰腺癌的生长、浸润及转移起重要作用。 |
| 英文摘要: |
| Objective: To study the mRNA and protein expression levels of osteopontin in pancreatic cancer and the relationship
with clinic pathological features. Methods: Amount of 50 cases of pancreatic cancer surgical specimens and 20 cases of adjacent normal
tissue were studied. The OPN mRNA and protein expression levels were detected by RT-PCR and immunohistochemistry respectively.
Results: RT-PCR analysis showed that the OPN mRNA were highly expressed in pancreatic tissues (45/50, 90 % ), which was
significantly higher than in adjacent normal tissues (3/20, 15 %) (P<0.01). Immunohistochemistry analysis demonstrated that the OPN
protein was high expressed in pancreatic tissues (43/50, 86 %), which was significantly higher than in adjacent normal tissue (6/20, 30 %)
(P<0.01). The expression levels of OPN in pancreatic cancer were obviously associated with lymph node metastasis (P<0.05).
Conclusion: The OPN was overexpressed in pancreatic cancer, which maybe played important roles in the growth, invasion and
metastasis of cancer. |
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