| 吴大畅王丽新张翠丽辛毅△.壳寡糖对乳酸杆菌体外生长的影响[J].,2012,12(5):849-854 |
| 壳寡糖对乳酸杆菌体外生长的影响 |
| Effect of Chito-Oligosaccharide on Growth of Lactobacillus in Vitro |
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| DOI： |
| 中文关键词: 壳寡糖 乳酸杆菌 体外培养 生长曲线 |
| 英文关键词: COS Lactobacillus In vitro culture Growth curve |
| 基金项目: |
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| 摘要点击次数: 952 |
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| 中文摘要: |
| 目的：研究壳寡糖对乳酸杆菌体外生长的影响。方法：将乳酸杆菌MRS 培养基中的葡萄糖替换为壳寡糖及向原培养基中加
入适量的壳寡糖，通过测定OD 值比较乳酸杆菌的生长状况。结果：以壳寡糖完全代替葡萄糖的培养基中乳酸杆菌的生长状况不
如MRS 培养基，而少量壳寡糖与葡萄糖协同的培养条件使乳酸杆菌的生长适应期明显减短，促进其生长。结论：乳酸杆菌对壳寡
糖的利用不如对葡萄糖的利用，1g/l 壳寡糖与葡萄糖协同作用时可明显缩短乳酸杆菌的生长适应期，促进细菌生长。 |
| 英文摘要: |
| Objective: To investigate the effect of Chito-Oligosaccharide （COS）on the growth of Lactobacillus in vitro. Methods:
Replace glucose with COS in Lactobacillus medium and apply moderate COS to the original medium. Lactobacillus growth was detected
by measuring its OD value. Results: Lactobacillus growth in the original medium is better than that in the medium of COS replaced for
glucose, and the lag phase in the medium adding a little COS is shorter than the original medium, promote the multiplication of Lactobacillus
in vitro. Conclusion: Glucose is better than COS for the growth of Lactobacillus. The synergistic action of COS and glucose can
shorten the growth lag phase and promote the multiplication of Lactobacillus with 1g/l COS . |
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