| 徐强1 董大海1 缪珊3 李金翠4 高琨2 高丽莉2 侯顺利2
左晶2 刘红2 闫文2 杨银书2 卢娟2 徐文2△.锰对PC12 细胞的增殖抑制与凋亡研究[J].,2012,12(5):836-839 |
| 锰对PC12 细胞的增殖抑制与凋亡研究 |
| Oxidative Stress Mechanism of Manganese-treated PC12 Cell Line |
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| DOI： |
| 中文关键词: 锰 鼠嗜铬神经瘤细胞（PC12） 氧化应激 凋亡 |
| 英文关键词: Manganese Pheochromocytoma(PC12) Oxidative Stress Apoptosis |
| 基金项目:甘肃省自然科学基金课题（1010RJZA058） |
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| 中文摘要: |
| 目的：研究锰作用下PC12 细胞的增殖抑制作用与凋亡相关的形态学、生化指标改变。方法：用200,400,600,800μmol/L
MnCl2 的培养液，分别作用对数生长期PC12 细胞1,2,3,4d 后，用MTT 筛选锰的细胞毒性剂量；透射电镜观察细胞形态学变化；琼
脂糖凝胶电泳检测MnCl2 对PC12 细胞基因组DNA 的影响。结果：MTT 实验显示200-800μmol/L MnCl2 作用4 天对PC12 有显
著的抑制作用，呈剂量和时间依赖趋势,600μmol/L MnCl2 作用4d 对PC12 的抑制率可达50%以上。600μmol/L MnCl2 作用4d 电
镜可见细胞凋亡，同样条件下细胞DNA 碎片化。结论：PC12 细胞在锰作用下发生增殖抑制，原因是锰诱导PC12 细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the apoptosis effect of Mn on the PC 12 cell line by detecting the cell morphology and
biochemical changes. Methods: PC12 cells in logarithm period incubated in medim with 200, 400, 600, 800μmol/L manganese(MnCl2)
for 1day,2 days, 3 days,4days respectively; The cell viability was detected by MTT [3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyl tetrasoliumBromide];
Morphological changes of PC12 cells was investigated by transmisssion electron microscope; Agarose gel electrophoresis
was used to detect the genomic DNA of Mn-treated PC12 transmisssion electron microscope as well as biochemical hallmark of DNA
fragments. Results: The results of MTT revealed that manganese of different Concentrations (MnCl2200,400,600,800μmol/L) could
suppress the proliferation of PC12 cells in dose and time-dependent manner. The cell inhibited ratio at the fourth day in 600μmol/L
MnCl2 culture medium approached 50% or more. In the same condition apoptosis was observed in cells. Conclusion: Mn has generated
apoptosis which induced proliferation arrested of PC12 cells. |
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