| 陈海英1,2 高连如1△ 张宁坤1 杨明3 王志国1.脐带华通胶间充质干细胞向Flk1 阳性细胞分化的研究*[J].,2012,12(4):645-648 |
| 脐带华通胶间充质干细胞向Flk1 阳性细胞分化的研究* |
| Induce Mesenchymal Stem Cells from Human Umbilical Cord Wharton'sJelly Differentiate into Flk1-positive Cells |
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| DOI： |
| 中文关键词: 脐带 华通胶 间充质干细胞 Flk1 心血管前体细胞 2- 巯基乙醇 |
| 英文关键词: Umbilical cord Wharton's jelly Mesenchymal stem cells Flk1 Cardiovascular progenitors 2-mercaptoethanol |
| 基金项目:国家863 计划资助课题（No：2006AA02Z469） |
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| 中文摘要: |
| 目的：诱导脐带华通胶间充质干细胞向Flk1 阳性细胞分化。方法：胶原酶法分离培养脐带华通胶间充质干细胞，第3 代细胞
以含2- 巯基乙醇的分化培养基培养，应用RT-PCR 和流式细胞仪从mRNA 和蛋白水平检测Flk1 阳性细胞分化水平。结果：脐带
华通胶间充质干细胞Flk1mRNA 及蛋白表达极低，分化培养基培养后表达上调，48h 达高峰(P<0.05)，之后表达降低。结论：2- 巯
基乙醇可诱导脐带华通胶间充质干细胞向Flk1 阳性细胞分化，为从中分选Flk1 阳性细胞进行进一步研究提供了依据。 |
| 英文摘要: |
| Objective: Induce mesenchymal stem cells from human umbilical cord Wharton's jelly differentiate into Flk1-positive
cells. Methods: Mesenchymal stem cells from human umbilical cord Wharton's jelly (WJ-MSCs) were isolated by collagenase digestion
and passage 3 cells were cultured by differentiation medium contained 2-mercaptoethanol, Flk1 mRNA were examined by RT-PCR, Flk1
protein were analyzed by flow cytometry. Results: Low expression of Flk1 mRNA and Flk1 protein were detected in WJ-MSCs. Flk1
expression increased after cultured by differentiation medium, and reached maximum after 48h culture, then declined. Conclusions:
Mesenchymal stem cells from human umbilical cord Wharton's jelly could be induced and differentiated into Flk1-positive cells by
2-mercaptoethanol, which can be used to select Flk1-positive cells in WJ-MSCs. |
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