| 白净1 张效林2 梁振洋2 孙莹2 冯雪瑶2 韩雅玲2△.ABCB1 基因单核苷酸多态性rs2235048 与氯吡格雷抵抗的相关关系[J].,2012,12(4):601-604 |
| ABCB1 基因单核苷酸多态性rs2235048 与氯吡格雷抵抗的相关关系 |
| The Association between ABCB1 Gene rs2235048 Single NucleotidePolymorphism and Clopidogrel Resistance |
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| DOI： |
| 中文关键词: 氯吡格雷抵抗 ABCB1 单核苷酸 多态性 |
| 英文关键词: Clopidogrel resistance ABCB1 Single nucleotide Polymorphism |
| 基金项目:军队临床高新技术重大项目(2010GXJS001) 国家自然科学基金青年科学基金项目（81100135） |
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| 中文摘要: |
| 目的：探讨ABCB1 基因单核苷酸多态位点rs2235048 在中国汉族人群中的分布及其与氯吡格雷抵抗（Clopidogrel
resistance，CR） 发生的相关关系。方法：采用光学比浊法测定20μmol/L ADP 诱导的残余血小板聚集率（Residual platelet
agglutination，RPA）。当RPA≥70%时，即为CR。所有入选患者分为CR 组和氯吡格雷非抵抗组（Non-clopidogrel resistance，NCR）。
采用焦磷酸测序法测定ABCB1 基因rs2235048 单核苷酸多态位点在CR 组和NCR 组的基因型及等位基因分布频率。结果：
ABCB1 基因rs2235048 多态在CR 组和NCR 组基因型分布频率符合Hardy-Weinberg 平衡。在CR 组和NCR 组中，ABCB1 基因
rs2235048 多态的基因型频率分布无统计学差异（P= 0.527，X2=1.281）；T、C 等位基因频率在两组间分布频率也没有统计学差异
（P= 0.740，OR=0.958，95%CI=0.742~1.236）。结论：对PCI 术后服用氯吡格雷的冠心病患者进行分析发现，ABCB1 基因单核苷
酸多态位点rs2235048 与冠心病患者CR 的发生无相关关系。 |
| 英文摘要: |
| Objective: To investigate the frequnency of gene polymorphism in rs2235048 of ATP-binding cassette subfamily B
member l transporter (ABCB1) gene and to elucidate the correlation between this polymophism and the occurrence of clopidogrel
resistance(CR). Methods: Clopidogrel response was assessed by posttreatment ADP 20μmol/L-induced platelet aggregation. Clopidogrel
resistance was defined by RPA (RPA≥70%), and all the enrolled 800 coronary artery diseases (CAD) patients after percutaneous
coronary intervention (PCI) were divided into CR group and Non-CR (NCR) group. Genotypes were determined by polymerase chain
reaction (PCR) and the sequences of rs2235048 of ABCB1 gene were analyzed. Results: The genotype frequencies in rs2235048 of
ABCB1 gene conformed well to the Hardy-Weinberg equilibrium for both CR group and NCR group. Between the two groups, the
genotype and allele frequency was similar (P=0.527 and 0.740, respectively). Conclusions: Based on the genetic analysis in patients after
PCI with clopidogrel therapy, there was no correlation between ABCB1 rs2235048 polymorphism and clopidogrel resistance. |
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