欢迎访问《现代生物医学进展》编辑部！

目的：研究白藜芦醇（resveratrol，Res）对肝癌细胞LM3 周期及凋亡的影响并探讨其可能的分子机制。方法：分别用50、100、 150、200μmol/L 浓度的Res 作用LM3 肝癌细胞24、48 和72h，CCK-8 测定Res 对细胞的增殖作用；分别以相同剂量的DMSO 及无处理的LM3 细胞为随机和空白对照，观察150μmol/L Res 作用72 小时后对肿瘤细胞周期及凋亡的影响；Real time-PCR 及 Western blot 分析Res 对LM3 细胞中Bak 和Bcl-2 mRNA 及蛋白表达的影响。结果：Res 体外能明显抑制肝癌细胞LM3 的生长增殖，在一定范围内呈现作用浓度（F=101.183，P＜0.001）和时间依赖性（F=192.371，P＜0.001）；150μmol/L Res 作用72 小时后能显著减缓肝癌细胞LM3 的周期转换（P＜0.001），并诱导明显的细胞凋亡效应（P＜0.001）；进一步的检测发现Res 作用LM3 细胞后，Bak mRNA（P=0.002，0.007）及蛋白（P = 0.004，0.01）表达增强，而Bcl-2 mRNA（P=0.027，0.007）及蛋白（P = 0.001，0.001）表达出现显著下降。结论：Res 可能通过对Bak 及Bcl-2 表达的调节来抑制肝癌细胞LM3 的增殖，并诱导其细胞凋亡。

英文摘要:

Objective: To investigate the effect of resveratrol (Res) on cell cycle and apoptosis of hepatocellular carcinoma LM3 cells and the possible molecular mechanisms. Methods: Cell counting kit-8(CCK-8) assay was used to examine the effect on cell proliferation of hepatocellular carcinoma LM3 cells treated by Res with the concentration of 50μmol/L for 24 and 72 hours; LM3 cells were treated by DMSO or no treatment as randomized and blank control, and then were detected. The expression of Bak and Bcl-2 mRNA and protein in LM3 cells treated by Res were evaluated by Real-time PCR and western blot, respectively. Results: Res inhibited the proliferation of LM3 cells in time-dependent manner（F=101.183，P＜0.001）and concentration-dependent manner（F=192.371，P＜0.001）in vitro. It was found that Res could accelerate cells cycle transition and induce apoptosis after treatment by Res at 150μmol/L for 72 hours. The expression of Bak mRNA (P=0.002，0.007) and protein （P = 0.004，0.01）were obviously up-regulated on treated LM3 cells, while the expression of Bcl-2 mRNA（P=0.027，0.007）and protein（P = 0.001，0.001）decreased remarkably. Conclusion: Res can not only inhibit proliferation of LM3 cells but also induce cellular apoptosis through accommodating the expression of Bak and Bcl-2.

查看全文查看/发表评论下载PDF阅读器

关闭