| 田刚1 樊明新1 陈祖容2 徐英1 杨江河1 卢宁3 李华1 张翼冠4.ID1对大鼠C6细胞侵袭转移能力的影响[J].,2011,11(21):4047-4053 |
| ID1对大鼠C6细胞侵袭转移能力的影响 |
| The effect of ID1 on invasive ability of C6 |
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| DOI： |
| 中文关键词: NF-КBp65 MMP-9 ID1 侵袭力 |
| 英文关键词: NF-КBp65 MMP-9 C6 Invasive ability |
| 基金项目:国家自然科学基金资助( 30901990) |
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| 中文摘要: |
| 目的：观察ID1对大鼠胶质瘤细胞（C6 细胞）侵袭转移能力的影响。方法：以C6 细胞为研究对象，ID1 siRNA 通过脂质体转
染C6 细胞，western blot 检测C6 细胞中ID1、P-NF-КBp65 蛋白的表达、Elisa 法检测细胞培养上清MMP-9 的含量，Transwell 小
室观察成的转移侵袭能力。结果：干扰ID1后能显著降低C6 细胞中P-NF-КBp65 蛋白、细胞培养上清中MMP-9的含量(P<0.01)，
降低C6 细胞的转移侵袭能力(P<0.01)。结论：干扰ID1 能抑制C6 细胞的转移侵袭，其机制可能与抑制C6 细胞中P-NF-КBp65
蛋白、MMP-9的表达有关。 |
| 英文摘要: |
| Objective: To study the effect of ID1on invasive ability of C6. Methods: C6 cells were divide into control, non sense ,
ID1 siRNA groups. The effects of ID1siRNA on C6 cells invasive ability in vitro was measured by Boyden chamber invasive models.
Contents of ID1, NF-КBp65, MMP-9 protein were detected by western blot and ELISA methods, respectively. Result: In ID1 siRNA
group, contents of NF-КBp65 and MMP-9 protein were decreased markedly(P<0.01), the invasive ability was decreased markedly(P<0.
01), too. Conclusion: ID1 could increase the invasive ability of C6 cell. |
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