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目的：研究早期吸入不同浓度布地奈德对哮喘大鼠气道炎症和气道重构的干预情况。方法：32 只Wistar 大鼠随机分为4 组：A对照组8只，B卵蛋白（OVA）致哮喘组8 只，C卵蛋白致哮喘后吸入低浓度布地奈德治疗组8只，D卵蛋白致哮喘后吸入高浓度布地奈德治疗组8只。分别测定各组大鼠血中肿瘤坏死因子-α（TNF-α）及肺泡灌洗液（BALF）中内皮素-1（ET-1）的水平，计数BALF中细胞总数及分类。各组大鼠行肺组织切片HE染色，再行胶原染色、免疫组化NGF、TGF-β1染色，借助计算机图象分析软件测量单位气道面积炎性细胞数目，基底膜周径（Pbm）、平滑肌面积（WAm）、气道内壁面积（WAi）、胶原面积（Wcol），NGF 及TGF-β1阳性信号积分吸光度。结果：B组BALF中细胞总数、嗜酸细胞分类及TNF-α、ET-1 水平与A 组比较均明显增加，差异有统计学意义（P<0.01）,C组及D组较B组均明显降低，差异有统计学意义（P<0.01）。B组NGF及TGF-β1的表达、气道壁炎性细胞计数、气道内壁面积、平滑肌面积、胶原面积与A 组比较均明显增加，差异有统计学意义（P<0.01）,C 组及D 组与B 组比较均明显降低，差异有统计学意义（P<0.01），C 组及D 组与A 组比较差异有统计学意义（P<0.05，P<0.01），C 组与D 组差异均有统计学意义（P<0.05，P<0.01）。结论：早期吸入不同浓度的布地奈德均可明显抑制气道炎症和气道重构，高浓度较低浓度对气道炎症和气道重构的影响更明显。

英文摘要:

Objective: To investigate the effect of inhaled different concentrations of budesonide in early phase on the airway inflammation and remodeling in asthmatic rats. Methods: Thirty-two rats were randomly divided into 4 groups: group A (n=8,control with saline), groupB (n=8,asthma with OVA),group C (n=8, asthma with OVA treated with low concentrations of budesonide in early phase), group D (n=8, asthma with OVA treated with high concentrations of budesonide in early phase). The bronchoalveolar lavage fluids (BALF) were classification in BALF studied in each group . the pathologic alteration of the bronchi and lung tissue was observed by HE staining. Expression of NGF and TGF-β1 were detected by immunohistochemistry, collagen deposition by Masson staining. The Morphological parameters including the number of inflammatory cell per unit airway area, bronchial basement membrane perimeter (Pbm), smooth muscle area（WAm）, inner airway area（WAi）, collagen area（Wcol）by computer image analysis software. Results: The total cell counts and the percentage of EOS in BALF the level of TNF-α, ET-1 increased compared with group A（P<0.01）, group C and D reduced significantly compared with group B（P<0.01）. The expression of NGF and TGF-β1, inflammatory cell counts around bronchus, airway smooth muscle hypertrophy ,the collagen deposition of reticular basement, airway inner wall area were significant higher than those groupA（P<0.01）, the data in group C and D were significant lower than those in group B（P<0.01）, there were apparent difference between group C and group D compared with group A (P<0.05,P<0.01). The difference between group C and group D.（P<0.05,P<0.01） Conclusion: Inhaled different concentrations of budesonide in early phase could apparently inhibit airway inflammation and remodeling, high concentrations of budesonide than low concentrations.

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