| 叶玲玲刘红李世崇刘兴茂陈昭烈.诱导表达p27对HEK293细胞生长代谢的影响[J].,2011,11(21):4001-4004 |
| 诱导表达p27对HEK293细胞生长代谢的影响 |
| Effects of p27 Inducible-Expression on the Growth and Metabolismof HEK293 cells |
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| DOI： |
| 中文关键词: HEK293细胞 细胞周期 代谢 p27 诱导表达 |
| 英文关键词: HEK293 cells Cell cycle Metabolism p27 Inducible-expression |
| 基金项目: |
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| 中文摘要: |
| 目的：研究诱导表达p27对HEK293细胞生长和代谢的影响。方法：将pTet-on 载体和响应于Dox的p27诱导表达载体共转
染HEK293细胞，随机挑选单克隆细胞株。以细胞周期分布和活细胞密度为主要观察指标，考察稳定转染的细胞在Dox诱导下的
细胞生长；以Qglc、Qlac 和Qgln为主要观察指标，考察转染细胞在Dox诱导下的细胞代谢。结果：p27 基因的表达使HEK293 细
胞的增殖速度显著降低，G1期细胞比例显著升高，葡萄糖消耗和乳酸生产减少。结论：诱导表达p27基因是对HEK293细胞进行
G1期阻滞的一种有效策略。 |
| 英文摘要: |
| Objective: To investigate the effect of p27 inducible-expression on the growth andmetabolismofHEK293 cells. Methods:
The vector pTet-on and Dox-responsive p27-expressing vector were co-transfected into HEK293 cells and individual clones were isolated
randomly. Cell growth and metabolism were assessed by cell-cycle distribution and viable cell density, Qglc, Qlac and Qgln as indexes,
respectively. Results: The expression of p27 increased percentage of G1 cells, reduced consumption of glucose and production of lactate
of HEK293 cells. Conclusions: The inducible-expression of p27 is an effective strategy for G1-phase arrest of HEK293 cells. |
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