| 张儒有郑永日△ 郭薇胡韶山林宏伟沈大伟.脑挫裂伤致miR-9 升高促进神经干细胞分化[J].,2011,11(20):3888-3890 |
| 脑挫裂伤致miR-9 升高促进神经干细胞分化 |
| Up-regulation of miR-9 Regulates Adult Neural Stem Cell Proliferationand Differentiation in Traumatic Brain Injury |
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| DOI： |
| 中文关键词: miR-9 脑挫裂伤 神经干细胞 增殖 分化 |
| 英文关键词: miR-9 Neural stem cells Proliferation Differentiation |
| 基金项目:黑龙江省教育厅基金（11521135） |
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| 中文摘要: |
| 目的：探讨颅脑损伤后miR-9 表达的变化和对神经干细胞分化和增值的影响，为颅脑损伤后神经功能修复治疗提出新的思
路。方法：通过RT-PCR 技术检测miR-9 在挫裂伤脑组织中的表达情况；培养胚胎来源神经干细胞，并通过免疫荧光鉴定神经干
细胞及其分化；转染miR-9 后，通过MTT 测定神经干细胞的增殖情况，和流式细胞仪检测分化神经元所占比例。结果：miR-9 在挫
裂伤脑组织中表达显著上升。对神经干细胞过表达miR-9 可显著促进细胞增殖，并诱导分化成神经元。结论：脑挫裂伤时miR-9
显著升高，并具有着促进神经干细胞增值和诱导分化的作用，可为伤后神经功能修复提供新的治疗方法。 |
| 英文摘要: |
| Objective: To investigate the expression of miR-9 in traumatic brain injury and the impact of miR-9 for proliferation
and differentiation of neural stem cells; to propose new ideas for neurological functions repair in traumatic brain injury. Methods: The
expression of miR-9 was detected by RT-PCR in traumatic brain tissues; the embryonic neural stem cells were cultured and identified by
immunofluorescence; After miR-9 transfection, the proliferation of neural stem cells were measured by MTT, and the proportion of differentiated
neurons were measured by flow cytometry. Results: The expression of miR-9 was significantly up-regulated in traumatic brain
injury. Overexpressing miR-9 can significantly promote neural stem cells proliferation and induced them to differentiate into neurons.
Conclusion: Overexpressing of miR-9 could promote neural stem cell proliferation and induce differentiation, that provide new treatments
for the restoration of neurological function after traumatic brain injury. |
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