| 刘佳1 杨晓彤1△ 杨庆尧2.流式细胞术BrdU/DNA 双染法测定云芝糖肽
诱导的Molt-4 细胞周期阻滞[J].,2011,11(20):3826-3829 |
| 流式细胞术BrdU/DNA 双染法测定云芝糖肽
诱导的Molt-4 细胞周期阻滞 |
| Polysaccharopeptides Derived from Trametes Versicolor Induce Cell CycleRetardarce on Molt-4 Cells Determined by Flow Cytometry BrdU/DNABivariate Immunostaining Analysis |
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| DOI： |
| 中文关键词: 云芝糖肽（PSP） 细胞周期 Molt-4 流式细胞术BrdU/DNA 双染法 |
| 英文关键词: PSP cell cycle Molt-4 BrdU/DNA bivariate immunostaining analysis |
| 基金项目: |
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| 中文摘要: |
| 目的：探究云芝糖肽(PSP) 对人急性淋巴母细胞白血病Molt-4 细胞周期的影响。方法：采用流式细胞术BrdU/DNA 双染法获
得各时相细胞分布状况和细胞周期的动力学参数。结果：0.1 mg/mlPSP 处理12 h 后，G2/M 期细胞百分比由对照组的11.09 %减少
至3.69 %。DNA 合成时间由12.10 h 延长至108.40 h。24 h 处理组中，S 期细胞百分比由对照组的43.29 %增加至67.26 %，而
G0/G1 期和G2/M 期细胞百分比均减少，G0/G1 期细胞百分比由对照组的37.47 %减少至27.43 %，G2/M 期细胞百分比由对照组
的19.24 %降低至5.31 %。DNA 合成时间更是由11.95 h 延长至114.52 h。结论：PSP 对人急性淋巴母细胞白血病Molt-4 细胞周期
的阻滞作用在于S 期，该作用与DNA 合成抑制有关。 |
| 英文摘要: |
| Objective: To investigate the cell cycle inhibition effects of Polysaccharopeptides derived from Trametes versicolor
(PSP) on human acute leukemia Molt-4. Methods: Flow cytometry BrdU/DNA bivariate immunostaining method was used to detect the
distribution of each phase and cell cycle kinetic parameters. Results: After treatment by 0.1 mg/ml PSP for 12 h, the percentage of G2/M
phase cells were dropped from 11.09% (control) to 3.69%. DNA synthesis time extended from 12.10 h to 108.40 h. In 24 h treatment
group , the percentage of S phase cells increased from 43.29 %(control) to 67.26 %, while the percentage of G0/G1 phase and G2/M
phase cells were all reduced, G0/G1 phase cells decreased from 37.47 % (control) to 27.43 %, G2/M phase cells reduced from 19.24 %
(control ) to 5.31 %. DNA synthesis time was prolonged from 11.95 h to 114.52 h. Conclusions: PSP induce cell cycle retardarce in S
phase on human leukemia Molt-4 cells,and related to inhibition of DNA synthesis. |
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