| 潘逸茹1 喻红之1 薛惠君1 陆元善2.人脂联素重组克隆载体构建[J].,2011,11(20):3805-3808 |
| 人脂联素重组克隆载体构建 |
| Reconstruction of Eukaryotic Expression Vector Containing HumanAdiponectin G |
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| DOI： |
| 中文关键词: 脂联素 慢病毒载体；构建；表达 |
| 英文关键词: Adiponectin Vector Construction |
| 基金项目:院级基金资助（H-425） |
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| 中文摘要: |
| 目的：构建含有人脂联素(adiponectin, ad)基因的重组克隆载体pEGFP-N1-AD，为进一步研究AD 与脂质代谢及代谢综合征
关系奠定基础。方法：根据Gene-Bank 中已经公布的AD 设计引物，从含有目的基因的质粒克隆模板中，利用PCR 方法钓取目的
基因。将AD 基因克隆到真核表达载体pEGFP-N1 中。酶切、PCR、及测序鉴定。结果：PCR、酶切及测序鉴定证实目的基因正确克
隆至真核表达载体pEGFP-N1 中，测序结果与Gene-Bank 报道一致。结论：成功构建了AD 重组克隆真核表达载体。 |
| 英文摘要: |
| Objective: To construct the vector (pEGFP-N1-AD) containing human adiponectin （AD）gene so as to investigate the
effects of adiponectin in the lipid metabolism and the syndrome of metabolism. Methods: AD gene was amplified from the plasma by
polymerase chain reaction (PCR) technique whose primer was designed according to the announced sequences of AD gene in gene bank,
then cut by the endonucleases and directionally cloned into the pEGFP-N1 vector and analyzed by endonuclease cutting,PCR and gene
sequencing analysis. Results: Through PCR, endonuclease cutting and gene sequencing,the target gene was verified to be correctly
cloned to vector pEGFP-N1. Conclusion: Recombinant eukaryotic expression vector containing human adiponectin protein was
successfully constructed. |
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