| 方茂楠苏晓荣卫静谢利芳刘涛.胰岛素对β细胞胰岛素分泌的反馈影响[J].,2011,11(19):3706-3708 |
| 胰岛素对β细胞胰岛素分泌的反馈影响 |
| Freeback Effects of Insulin on Insulin Secretion in Beta Cells |
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| DOI： |
| 中文关键词: 胰岛素 βTC-3 细胞 C 肽 酶联免疫法 |
| 英文关键词: Insulin βTC-3 cell C-peptide ELISA |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨不同浓度外源性胰岛素在不同浓度葡萄糖情况下对βTC-3 细胞胰岛素分泌的影响。方法：取对数生长期的
βTC3 细胞分三组，即低糖组、中糖组、高糖组（葡萄糖浓度分别取1.0mmol/L、3.0mmol/L、20.0mmol/L）。每组分0、5、10、15、100、
500、5000 和50000μU/ml 胰岛素八个亚组（其中0μU/ml 作为对照组）。刺激10 分钟后取上清液测C 肽。结果：在高糖组中，C 肽
分泌量无明显差异；在中糖组中，10μU/ml 和15μU/ml 两组相对对照组C 肽分泌量显著增加，50000μU/ml 组C 肽分泌量则相对
对照组出现减少，其余3 个亚组无明显改变；在低糖组中，C 肽分泌量除5000μU/ml 组减少外，其它亚组C 肽分泌量无明显差异。
结论：胞外胰岛素在适宜葡萄糖浓度时，对βTC3 细胞胰岛素分泌的反馈影响呈剂量依赖关系。 |
| 英文摘要: |
| Objective: To investigate the effects of different concentrations of glucose and insulin on Glucose-stimulated insulin
secretion of β TC-3 cells. Methods: β TC-3 cells in exponential phase of growth were divided into three groups: low sugar group
(1.0mmol/L), middle sugar group (3.0mmol/L), and high sugar group (20.0mmol/L). Each group were subdivided into eight subgroups,
with different concentrations of insulin(0, 5, 10, 15, 100, 500, 5000 and 50000m U/ml), taking the subgroup of 0 m U/ml as control. After
stimulated for 10 minutes, supernate of each subgroup were collected to detect the c-peptide. Results: In high sugar groups, secretions of
C-peptide were similar with no obvious difference. In middle sugar groups, secretion of C-peptide in 10m U/ml and 15m U/ml subgroups
were obviously increased, while that in 50000 mU/ml subgroup was reduced, compared with the control group; no obvious changes were
found in other three subgroups. In low sugar groups, secretion of C-peptide was reduced only in 5000mU/ml subgroup, and other
subgroups showed no obvious changes. Conclusion: When the extracellular insulin is in suitable glucose concentration, the effect of
insulin on glucose-stimulated insulin secretion is dose-dependent. |
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