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目的：探讨兔后交叉韧带（Posterior cruciate ligament，PCL）断裂对外侧胫骨平台组织学的影响。方法：48 只家兔膝关节随机配对为实验侧和对照侧造模，造模后第4、8、16、24 周各随机处死12 只，行外侧胫骨平台大体观察、HE 染色、免疫组化检测基质金属蛋白酶13 （matrix metalloproteinase-13，MMP-13）、基质金属蛋白酶抑制剂1 （Tisse inhibitor-1 of matrix metalloproteinase1, TIMP-1）表达。结果：①大体观察，随时间延长，实验组外侧胫骨平台软骨出现磨损，呈灰黄色，弹性差，骨赘形成。②组织学观察，随时间延长，胫骨平台软骨纤维化，细胞排列紊乱，簇聚细胞出现频率增加。③实验组MMP-13、TIMP-1 表达均高于对照组，有显著性差异，P＜0.05。④实验组MMP-13、TIMP-1 表达阳性率第4、8、16 周逐渐升高，24 周下降，各组比较有显著性差异，P＜0.05。结论：①兔膝关节PCL 断裂会引起外侧胫骨平台软骨退行性变，且该退变随着时间的推移逐渐加重。② MMP-13 与TIMP-1 在 PCL 断裂膝关节外侧胫骨平台中的表达呈现先高后低的变化规律，造成MMP-13 与TIMP-1 的失衡，加速软骨退变。③ MMP-13 与TIMP-1 表达增高提示MMP-13 与TIMP-1 可能参与了PCL 断裂后外侧胫骨平台软骨的退变过程。

英文摘要:

Objective: To explore the histological influence on lateral tibial plateaus which was caused by rupture of PCL. Methods: The modes of lateral tibial plateaus of 48 rabbits were matched in pairs randomly including experiment group which PCL were transacted and control group，at the 4th,8th,16th and 24th week, 12 rabbits were sacrificed randomly, then lateral tibial plateaus were observed in general and through HE staining and immunohistochemistry staining, the expression of matrix metalloproteinase-13 (MMP-13)and Tisse inhibitor-1 of matrix metalloproteinase1 (TIMP-1) was detected. Results: 1）. The observation in general showed that along with the increase of the time, lateral tibial plateaus of experiment group are gradually presented with abrasion, luidity, bad elasticity and formation of osteophytes. 2). Along with the increase of the time, the observation of histology showed fibering of lateral tibial plateau, meanwhile, there are abnormal cell disposition and increasingly clustered cell. 3). Expression of MMP-13 and TIMP-1 was higher in experimental group than that in control group, showing significant difference, P<0.05. 4）. In experimental group, the positive rate of expression of MMP-13 and TIMP-1 were increased gradually at the 4th,8th and 16th week, and decreased at 24th week, showing significant difference, P<0.05. Conclusions: 1). Rupture of PCL could cause the degeneration of the lateral tibial plateau. As time goes on, this condition will be aggravated. 2). The expression of TIMP-1 and MMP13 in lateral tibial plateau of ruptured PCL of knee tend to be low→high, causing the unbalance between MMP-13 and TIMP-1.3L, therefore, sped up the degeneration of the cartilage. 3). The increase of the expression of MMP-13 and TIMP-1 indicated that they may participate in the course of degeneration of the lateral tibial plateau cartilage.

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