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目的：通过检测成人急性髓性白血病中SOCS-1 基因表达水平及其甲基化水平，研究其在白血病发病中的作用。方法：运用甲基化特异性PCR（Methylation specific PCR，MSP）方法，对24 例急性髓性白血病患者和4 株白血病细胞株（Jurkat、Raji、U 937、 NALM 17），进行SOCS-1 基因甲基化水平的研究；同时运用Real-time PCR 法定量分析SOCS-1 基因表达水平。以10 例健康人为正常对照组。结果：24 例成人急性髓性白血病患者中，15 例有SOCS-1 基因甲基化（62.5%），而正常对照组无SOCS-1 基因甲基化（0％），二者有显著差异（P<0.05）；SOCS-1 基因甲基化组与无SOCS-1 基因甲基化组相比较，其SOCS-1 基因相对表达量明显减少（P﹤0.05）；与患者临床病理特征相结合比较，发现SOCS-1 基因的甲基化与患者年龄、性别和病程阶段无相关。4 株白血病细胞株中，Jurkat 和U 937 表现有SOCS-1 甲基化（50％），Raji 和NALM 17 无SOCS-1 甲基化，前者SOCS-1 基因表达量较后者也明显降低（P<0.05）。结论：SOCS-1 基因在成人急性髓性白血病中甲基化水平明显增高，且SOCS-1 基因甲基化后表达水平受到抑制，提示SOCS-1 基因及其甲基化在急性髓性白血病的发生发展中可能具有一定作用。

英文摘要:

Objective: To investigate the role of SOCS-1 (Suppressor of Cytokine Signalling-1) in tumorigenesis of adult acute myeloid leukemia （AML）by detecting the methylation state and expression of SOCS-1 gene. Methods: A total of 24 patients with AML, 10 healthy volunteers and 4 cell lines (Jurkat, Raji, U 937, NALM 17) were chosen. The methylation state of SOCS-1 in samples and cell lines were detected by using methylation-specific polymerase chain reation (MSP), and the expression of SOCS-1 was detected by using Real-time PCR. Results: The aberrant methylation of SOCS-1 was 62.5％（15/24）in primary patients with AML. In contrast, there was no aberrant methylation of SOCS-1 gene in normal samples (P<0.05). The expression of SOCS-1 in AML patients with aberrant methylation of SOCS-1 gene was lower than that in AML patients without aberrant methylation of SOCS-1 gene (P<0.05). There was no relation between the methylation of SOCS-1 gene and the clinicopathological data (such as sex, age and tumor stage). Conclusion: The methylation of SOCS-1 gene in AML was higher than that in control group significantly (P<0.05), and aberrant methylation strongly correlates with reduced expression. SOCS-1 gene may play an important role in tumorigenesis of AML.

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