| 陈晓明1 游运辉2 罗卉2 贺立新3,4.来氟米特联合依那西普对佐剂性关节炎大鼠免疫功能的影响[J].,2011,11(17):3266-3269 |
| 来氟米特联合依那西普对佐剂性关节炎大鼠免疫功能的影响 |
| Effects of the Combined Use of Leflunomide with Etanercept on ImmuneFunction in Rat Adjuvant Arthritis |
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| DOI： |
| 中文关键词: 类风湿关节炎 来氟米特 依那西普 白细胞介素-1 肿瘤坏死因子 基质金属蛋白酶-3 |
| 英文关键词: Rheumatoid arthritis Leflunomide Etanercept IL-1 TNF MMP-3 |
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| 中文摘要: |
| 目的：研究来氟米特和依那西普联合使用对佐剂性关节炎（AA）大鼠的治疗作用及其可能的作用机制。方法：建立AA 大鼠
关节炎模型，分为正常对照组、模型组、来氟米特组、依那西普组、来氟米特联合依那西普配伍组；采用关节炎指数评分法评价大
鼠关节炎症程度，半定量RT-PCR 和放射免疫法检测滑膜组织及血清中IL-1β、TNF-α 表达水平，免疫组化方法检测滑膜组织中
MMP-3 含量。结果：①相较于AA 模型组，来氟米特组、依那西普组和配伍组中大鼠的AI 评分均显著下降（P<0.01)，其中以配伍
组关节炎指数为最低（P<0.05)。②模型组大鼠血清及滑膜组织的IL-1β 和TNF-α 水平明显高于正常对照组(P<0.01)，用药后各
组的IL-1β 和TNF-α 水平均有所下降，并以配伍组降低最为明显(P<0.01 或P<0.05)；③模型组大鼠滑膜组织MMP-3 表达阳性
密度显著高于正常对照组（P<0.01)，用药后各组的MMP-3 阳性密度降低(P<0.01)，其中配伍组下降程度明显高于来氟米特组和依
那西普组(P<0.01)。结论：来氟米特和依那西普联合使用可明显减轻AA 大鼠的关节炎症，降低血清和滑膜组织中IL-1β 和
TNF-α 水平，减少滑膜中MMP-3 的表达，疗效优于单独使用来氟米特或依那西普。 |
| 英文摘要: |
| Objective: To study the therapeutic effects of Leflunomide (LEF) combined with Etanercept on rat adjuvant arthritis
(AA), as well as the possible mechanism. Methods: Established the rat model of collagen-induced arthritis, and the rats were divided into
control group, AA model group, LEF group, Etanercept group and LEF+Etanercept group; Arthritis were evaluated by arthritis index,
and semi-quantitative RT-PCR, radioimmunoassay and immunohistochemistry were respectively used to detect the levels of IL-1β,
TNF-α and MMP-3 in synovium and serum. Results: ① The arthritis index of AA model group was significantly higher than those of
LEF group, Etanercept group and LEF+Etanercept group (P<0.01), and the arthritis index was significantly lowest in LEF+Etanercept
group (P<0.05). ② The levels of IL-1β and TNF-α in synovium and serum were notably higher in AA model group than those in control
group (P<0.01). After administration, the levels significantly decreased in the three groups (P<0.01), especially in LEF+Etanercept
group (P<0.05). ③The positive expression of MMP-3 in synovium of model group was significantly higher that that of control group.
(P<0.01). The positive expression of MMP-3 in each group was lowered after administration. Compared with LEF group and Etanercept
group, the positive expression of MMP-3 was significantly lower in LEF+Etanercept group (P<0.01). Conclusions: The combined use of
LEF and Etanercept can significantly relieve the arthritis syndrome in AA rats by decreasing the expression of IL-1β and TNF-α in synovium
and serum, as well as that of MMP-3 in synovium, which is better than LEF or Etanercept used separately. |
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