| 李争鸣周秀萍林梅李秀萍.口服卡介菌治疗实验性自身免疫性脑脊髓炎的研究[J].,2011,11(17):3258-3261 |
| 口服卡介菌治疗实验性自身免疫性脑脊髓炎的研究 |
| Studies on the therapeutic effect of oral BCG on experimental autoimmuneencephalomyelitis |
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| DOI： |
| 中文关键词: 多发性硬化 实验性变态反应性脑脊髓炎 卡介菌 免疫耐受 |
| 英文关键词: Multiple Sclerosis EAE BCG Immune Tolerance |
| 基金项目:湖南省教育厅课题资助项目（06C069） |
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| 中文摘要: |
| 目的：观察口服卡介菌对实验性变态反应性脑脊髓炎(EAE)的治疗效果。方法：制备EAE 大鼠模型，随机分为BCG 高、中、
低剂量组和PBS 对照组，每组各15 只，对大鼠治疗后的临床症状及病理组织学进行评估，提取脾脏淋巴细胞，流式细胞术检测T
淋巴细胞亚群，3H-TdR 掺入法检测淋巴细胞增殖能力。结果：BCG 组EAE 大鼠与对照组相比，临床症状减轻，发病时间延迟，
炎性细胞浸润数减少；急性期，口服BCG 各组CD4+、CD8+T 细胞的数量随剂量增加而增加，缓解期CD4+、CD8+T 细胞数量减少；
口服BCG 可促进EAE 大鼠T 淋巴细胞增殖能力；高、中剂量组上述变化均较其它分组明显。结论：口服BCG 可很好的诱导免疫
耐受，延迟EAE 发病，减轻炎症反应，改善临床症状。 |
| 英文摘要: |
| Objective: To investigate the therapeutic effect of oral BCG on experimental autoimmune encephalomyelitis. Methods:
The EAE rats were randomly divided into four groups: high dose BCG group, medium dose BCG group, low dose BCG group and PBS
control group. Clinical score and HE staining were respectively used for clinical symptom assessment and pathological examination.
Lymphocytes were extracted from spleen, then T-type lymphocyte subsets were tested by flow cytometry and lymphocyte proliferation
was tested by assay of 3H-TdR incorporation. Results: Compared with control group, clinical symptom was relieved, the disease onset
time was delayed, and the number of inflammatory cell infiltration was reduced in oral BCG group. The number of CD4+ and CD8+ T
lymphocytes of oral BCG group was increased in a dose-dependent manner during acute phase, but decreased during remission phase.
Oral BCG inhibited the proliferation of T lymphocytes. These changes in high and medium dose group were more obvious than those in
low dose and control groups. Conclusion: Oral BCG could induce immune tolerance, delay the onset time, relieve inflammatory response
and improve the clinical symptoms of EAE. |
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