| 施丽丹蔺剑李大伟.p150Sal2 在人卵巢癌细胞株中促进MX1 基因的表达[J].,2011,11(17):3216-3218 |
| p150Sal2 在人卵巢癌细胞株中促进MX1 基因的表达 |
| Induction of MX1 Expression by p150Sal2 in Human OvarianCancer Cell Line |
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| DOI： |
| 中文关键词: 卵巢癌细胞株 同源异型转录因子p150Sal2 MX1 基因 |
| 英文关键词: Ovarian cancer cell line homeotic transcription factor p150Sal2 MX1 gene |
| 基金项目:国家自然科学基金资助项目（NO. 30771212） |
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| 中文摘要: |
| 目的：研究p150Sal2 在人卵巢癌细胞株中对MX1 基因表达的影响。方法：将MX1 基因的启动子克隆至带有Luciferase 报
告基因的pGL3-basic 载体中，将其与p150Sal2 表达载体用PEI 介导的转染方法共转染至卵巢癌细胞株SKOV3，用双荧光素酶检
测系统检测MX1 启动子活性变化；将p150Sal2 表达载体转染至卵巢癌细胞株ES-2，用western blot 检测细胞中MX1 基因的表
达变化。结果：双荧光素酶检测系统检测MX1 启动子活性被p150Sal2 上调，western blot 检测转染p150Sal2 后细胞中MX1 表达
量增加。结论：p150Sal2 在人卵巢癌细胞株中促进MX1 基因的表达。 |
| 英文摘要: |
| Objective: To investigate the effect of p150Sal2 on MX1 gene expression of human ovarian cancer cell line. Methods:
Promoter of MX1 gene was cloned into pGL3-basic with luciferase reporter gene, and co-transfected with p150Sal2 expression plasmid
into SKOV3 cell line. The promoter activity was detected by using dual-luciferase reporter gene detection method. The MX1 expression
in ES-2 cells was assayed after transfection with p150Sal2 expression plasmid by western blot. Results: The activity of MX1 promoter
was up-regulated by p150Sal2 in SKOV3 cell line, and the western blot result showed increased expression of MX1 gene of ES-2 cell
line transfected with p150Sal2 expression plasmid. Conclusions: MX1 expression was induced by p150Sal2 in human ovarian cancer cell
line. |
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