| 张岩1 周志全1,2 刘晓昂1 李艳明1 赵永和1 王尚文1 李桢1△ 曾晓锋1.甲基苯丙胺中毒大鼠纹状体COX-2、PGE2、EP2 受体及小胶质细胞
表达的研究[J].,2011,11(17):3212-3215 |
| 甲基苯丙胺中毒大鼠纹状体COX-2、PGE2、EP2 受体及小胶质细胞
表达的研究 |
| The Expression of COX-2, PGE2, EP2 Receptor and Microgliain Rats' Striatum of Methamphetamine Intoxication |
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| DOI： |
| 中文关键词: 甲基苯丙胺 环氧合酶2 前列腺素E2 EP2 受体 小胶质细胞活化 |
| 英文关键词: Methamphetamine COX-2 PGE2 EP2 receptor Microglia activation |
| 基金项目:国家自然科学基金项目(NO30660202)；教育部春晖项目（NOZ2006-1-65003）；云南省科技厅项目（NO2006GH22）；
昆明医学院研究生创新基金（NO2009J01）；2010 年昆明医学院大学生创新性实验计划项目. |
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| 中文摘要: |
| 目的：通过研究COX-2、PGE2、EP2 受体及小胶质细胞在甲基苯丙胺中毒大鼠纹状体内的表达变化探讨甲基苯丙胺中毒大鼠
纹状体中COX-2/PGE2 系统与小胶质细胞活化之间的关系。方法：将40 只健康成年雄性SD 大鼠，随机分成对照组10 只和实验
组30 只(实验组分成三个亚组，分为末次给药后1 天组、2 天组和3 天组，n=10)。实验组给予10mg/kg 的MA 腹腔注射，对照组给
予同样剂量的生理盐水，每天注射两次，注射时间为8：00、20：00，连续注射4 天。分别于末次给药后的第1 天、第2 天、第3 天处
杀。用免疫组化技术对中毒大鼠纹状体(CPU)中COX-2、EP2 受体及Iba1（钙离子接头蛋白，小胶质细胞内一种特异性标记物）的表
达进行检测，并进行图像分析。另外，取大鼠的纹状体运用酶联免疫法检测PGE2 的含量。结果：COX-2、PGE2、EP2 受体及小胶质细
胞在各组均有表达。与对照组相比，实验组中：COX-2、PGE2、EP2 受体的1 天组表达均不同程度下降；2 天组中COX-2 表达水平
大幅度上升，PGE2、EP2 受体表达仍低于正常水平；3 天组COX-2 表达水平继续升高，而PGE2、EP2 受体表达趋于正常组水平。而小
胶质细胞表达水平则是三个实验组均高于正常组，且3 天组高于2 天组，2 天组高于1 天组。对照组与实验组有显著性差异（P<0.
05）。结论：COX-2/PGE2 系统与甲基苯丙胺中毒大鼠纹状体内小胶质细胞活化无明显相关性；COX-2 与甲基苯丙胺的神经毒性
有关。 |
| 英文摘要: |
| Objective: To investigate the relationship of COX-2/PGE2 system and the microglia activation in the methamphetamine
intoxication rats by investigating the expression of COX-2, PGE2, EP2 receptor and microglia in rats' striatum of methamphetamine
intoxication. Methods: 40 male Sprague-Dawley rats were randomly divided into control group(n=10) and experimental group
(dividing it into the first, second and third group after the last injection, n=10). Rats in the experimental group were intraperitoneal injected
with methamphetamine hydrochloride (10mg/kg), while those in the control group were injected with saline with the same volume (twice
a day at 8:00 and 20:00 respectively for 4 continuous days ). Then the protein expression of COX-2, EP2 receptor and Iba1 of intoxication
rats in striatum was detected by immunohistochemistry technique and the test results were analyzed by image; PGE2 was examined
by ELISA. Results: There was the expression of COX-2, PGE2, EP2 receptor and microglia in each group. The expression of COX-2,
PGE2 and EP2 receptor reduced by varying degree at the first day compared with that in the control group. The expression of COX-2 began
to rise and the expression of PGE2 and EP2 receptor still stay a low level at the second day. At the third day,the expression of COX-2
was higher than that in the control group and the expression of PGE2 and EP2 receptor became close to the control group. The expression
of microglia was higher than that in the control group in the 3 experimental group, and the 3d group was higher than the 2d group, the 2d
group was higher than the 1d group. Conclusion: There was no relationship between the COX-2/PGE2 system and the microglia activation;
There was a close relationship between COX-2 and MA neurotoxicity. |
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