| 杨江河张蓉李华修彬华闫志强刘绍明.姜黄素对糖尿病大鼠疼痛过敏的影响[J].,2011,11(13):2419-2422 |
| 姜黄素对糖尿病大鼠疼痛过敏的影响 |
| Effect of Curcumin on Hyperalgesia of Diabetic Rats |
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| DOI： |
| 中文关键词: 姜黄素 糖尿病 疼痛过敏 TNF-α STZ |
| 英文关键词: Curcumin Diabetes mellitus Hyperalgesia TNF-α STZ |
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| 中文摘要: |
| 目的：神经病理性痛是糖尿病最常见的并发症之一，本课题旨在探讨姜黄素对糖尿病大鼠痛觉过敏的影响及其分子机制。
方法：30 只雄性SD 大鼠随机分为对照组、糖尿病组和姜黄素治疗组，模型组和姜黄素治疗组利用腹腔注射链脲佐菌素（Streptozotocin，
STZ）制备大鼠糖尿病模型，定期检测大鼠血糖、饮食、体重等变化，治疗组于STZ 注射2wk 后定期灌服姜黄素，分别在
2wk 和4wk 后检测各组大鼠热痛敏和机械痛敏反应，在第4 wk 利用ELISA 分别检测各组大鼠脊髓背角TNF-α 表达变化。结果：
STZ 注射组大鼠2 周后出现血糖＞14mol/L，并且该模型具有高血糖、体重增长缓慢、多饮多食多尿的特点，符合I 型糖尿病特征，
痛行为测试结果显示糖尿病大鼠出现痛觉过敏，经过给予姜黄素灌服治疗后，痛觉过敏有所减轻，ELISA 分析结果表明糖尿病大
鼠脊髓背角TNF-α 表达升高，经过姜黄素治疗后TNF-α 表达有所下降。结论：成功制备STZ- 型糖尿病大鼠模型，经过姜黄素治
疗可以减轻糖尿病引起的疼痛过敏，姜黄素对糖尿病疼痛的治疗作用可能是通过降低大鼠脊髓背角TNF-α 表达实现的。 |
| 英文摘要: |
| Objective: The purpose of the study was to investigate the effects of curcumin on hyperalgesia of STZ-induced
diabetic rats. Methods: 30 male SD rats were randomly divided into three groups：control group (CN), diabetic mellitus group (DM) and
curcumin therapy group (DM+Cur). Streptozotocin (STZ) prepared in citrate buffer was injected intraperitoneally to induce DM. DM+Cur
group were treated with 100 mg/kg curcumin everyday. Mechanical withdrawal threshold （MWT） and thermal withdrawal latency
（TWL）of the rats were measured. The expressin of TNF-αin the spinal dorsal horn of rats was tested by ELISA. Results: The DM group
and DM+Cur group rats displayed hyperglycosemia, weight loss, polyphagia, polydipsia and polyuria. The MWT and TWL of DM group
rats decreased compared with CN group rats. After treated with curcumin, both MWT and TWL of DM+Cur group rats increased. The
expression of TNF-αin the spinal dorsal horn of diabetic rats was higher than that of CN group. After treated with curcumin, the level of
TNF-αwas reduced. Conclusions: Curcumin can and relieve diabetic neuropathic pain and reduce the expression of TNF-αin spinal cord
dorsal horn of STZ inducing diabetic rats. The expression of TNF-αin the spinal cord dorsal horn may participate diabetic pain. |
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