| 任利樊海宁邓勇王海久阳丹才让张鹏王展.乙肝病毒X 蛋白通过激活NF-κB 信号通路上调ABCG2 的表达[J].,2011,11(12):2247-2250 |
| 乙肝病毒X 蛋白通过激活NF-κB 信号通路上调ABCG2 的表达 |
| Hepatitis B virus X Protein Induces ABCG2 Expression by Activation ofNF-κB Signaling Pathway |
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| DOI： |
| 中文关键词: 乙型肝炎病毒X 蛋白 半转运蛋白ABCG2 核因子-κB 信号通路 肝癌 |
| 英文关键词: HBx ABCG2 NF-κB Signaling pathway Hepatocellular |
| 基金项目: |
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| 摘要点击次数: 723 |
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| 中文摘要: |
| 目的：研究乙肝病毒X 蛋白（HBx）通过核因子-κB (NF-κB)信号通路对半转运蛋白（ABCG2）的调节作用。方法：用特异性的
NF-κB 信号通路阻断剂PDTC 阻断NF-κB 信号通路，荧光双标激光扫描共聚焦显微镜观察L02 细胞系转染HBx 基因前后及
PDTC 加入前后NF-κB 信号通路的激活、失活情况，同时用Real-time PCR 和Western Blot 技术检测转染前后及PDTC 加入前后
ABCG2 在mRNA 及蛋白水平的表达变化。结果：以L02 细胞为参照，转染HBx 基因后的L02-HBx 细胞NF-κB 信号通路被激活，
ABCG2 mRNA 和蛋白水平分别增加3.62±0.15 和4.61±0.73 倍,差异有统计学意义（P<0.05）；PDTC 作用24h 后L02/HBx 细胞
NF-κB 信号通路阻断，ABCG2 mRNA 和蛋白表达分别为2.15±0.32 倍和2.37±0.55 倍，与未加入PDTC 作用的L02-HBx 细胞
相比均有统计学意义（P<0.05）。结论：NF-κB 信号通路是HBx 上调ABCG2 表达的途径之一。 |
| 英文摘要: |
| Objective: To investigate the effect of HBx on ABCG2 by NF-κB signaling pathway. Methods: NF-κB signaling
pathway blocker PDTC was blocked by PDTC. Laser scanning confocal microscopy was applied to detect the activation and deactivation
of NF-κB signaling pathway. Real-time PCR and Western Blot were used to detect the expression of ABCG2. Results: The NF-κB
signaling pathway of Lo2-HBx cells was activated after transfection with HBx gene compared with control Lo2 cells. The mRNA and
protein of ABCG2 in Lo2-HBx cells increased 3.62±0.15 and 4.61±0.73 times respectively. This difference had statistical significance
（P<0.05）. But the mRNA of ABCG2 decreased to 2.15±0.32,while the expression of ABCG2 reduced to2.37±0.55（P<0.05）, when the
NF-κB signaling pathway was blocked by 50μM PDTC for 24h respectively. Conclusion: NF-κB signaling pathway is one of the way
for HBx to up-regulate the expression of ABCG2. |
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