| 宋衍秋1 赵莉莉1 曹丽2 崔丽3 赵鸿铭1 毛用敏1△ 崔让庄1.慢性充血性心力衰竭大鼠模型的建立[J].,2011,11(12):2234-2237 |
| 慢性充血性心力衰竭大鼠模型的建立 |
| Establishment of Rat Models with Chronic Congestive Heart Failure |
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| DOI： |
| 中文关键词: 慢性心力衰竭 大鼠 动物模型 腹主动脉缩窄 |
| 英文关键词: Chronic heart failure Rat Animal models Abdominal aorta constriction |
| 基金项目:天津市卫生局基金项目（07KY01） |
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| 中文摘要: |
| 目的：探讨建立慢性充血性心力衰竭大鼠模型的方法。方法：采用腹主动脉缩窄法制备CHF 大鼠模型。于右肾动脉分支处上
方缩窄腹主动脉至0.6mm，同时设立假手术组及正常对照组。12 周后行一般情况观察，超声心动图及血液动力学检测心脏结构和
功能、计算心脏肥厚指数，HE 及Masson 染色检测心肌病理改变，综合评价CHF 大鼠心功能。结果：与假手术及正常对照组比较，
CHF 模型组术后12 周均出现进食减少、精神萎靡、少动、被毛无光；IVS、LVPW、LVM、LVEDD、LVESD、SV 显著增加，EF、FS 显
著降低；HR 显著增加；VSP、+dP/dtmax 显著降低，T-dP/dtmax 显著延长；LVEDP 显著升高，-dP/dtmax 绝对值显著降低；心脏湿重
及心脏肥厚指数均增加；病理结果表明CHF 模型组心肌细胞肥大、排列紊乱、心肌胶原纤维增多。结论：应用腹主动脉缩窄术可成
功制备CHF 大鼠模型。 |
| 英文摘要: |
| Objective: To establish rat models with chronic congestive heart failure. Methods: The CHF rats were prepared by
abdominal aorta constriction to 0.6mm. After 12 weeks, the observation of general condition, echocardiagraphy, hemodynamics
parameters, pathological HE staining and Masson staining were all performed to evaluate the cardiac function of CHF rats. Results: The
general condition of CHF rats were poor. Echocardiagraphy parameters showed IVS, LVPW, LVM, LVEDD, LVESD, SV increased, and
EF, FS decreased. Hemodynamics parameters showed HR, LVEDP, T-dP/dtmax increased, and LVSP, dP/dtmax decreased. The heart
wet weight and heart mass index of CHF rats were both increased. Pathological results showed there were hypertrophic and disordered
cardiomyocyte and proliferation of fibrous tissue. Conclusion: CHF rats were established by abdominal aorta constriction. |
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