| 高小寒1 魏蓓蓓1 田玲玲2 刘一浓1 秦明杰1 胡振华1 吴飞1.乳液法静电纺丝PLGA 组织工程缓释纤维的研究[J].,2011,11(12):2224-2226 |
| 乳液法静电纺丝PLGA 组织工程缓释纤维的研究 |
| Encapsulation of Proteins in PLGA fibers by Emulsion Electrospinning |
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| DOI： |
| 中文关键词: 乳液法 静电纺丝 PLGA 组织工程 葡聚糖 |
| 英文关键词: Emulsion Electrospinning Poly (lactic-co-glycolic acid) Tissue Engineering Dextran |
| 基金项目:“国家重大新药创制”针对生物技术药物的相关给药技术平台（2009ZX09310-007） |
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| 中文摘要: |
| 目的：研究Dextran 对蛋白药物的释放影响。方法：将模型蛋白BSA 溶解于多糖溶液中，通过W/O 乳液法静电纺丝制备缓
释纤维。采用MicroBCA 法测定该纤维体外释放行为，采用SEC-HPLC 检测制备前后蛋白的聚集程度，并与不含多糖的BSA 纤
维做对照。结果：添加Dextran 以后蛋白的包封率由52.68 %提高到63.92 %，第一天突释不大于药物载量的15 %，对蛋白单体的
保持达到85 %以上。结论：Dextran 可以改善一般组织工程纤维中蛋白药物的释放，提高蛋白药物在制剂、贮存、释放过程中的稳
定性，增加纤维的载药量。 |
| 英文摘要: |
| Objective: To investigate the effect of dextran on the releasing behavior of protein drug. Methods: BSA was dissolved
in dextran solution, then the fibers was prepared by W/O emulsion electrospinning. The release behavior of the fibers in vitro was
detected by MicroBCA method and BSA aggregation was determined by SEC-HPLC. The results were compared with the BSA fiber
without dextran. Results: Drug loading for the experiment group reached 63.92 % while control group was only 52.68 %, with first day
burst release less than 15 %, BSA monomer preserved more than 85 %. Conclusion: Dextran can improve release behavior of tissue
engineering fibers, increase drug loading and drug stability during preparation, storation and release process. |
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