| 毛竹君张慈安武峰魏品康.生半夏、南星水提物对人胃癌BGC823 细胞的侵袭力及
HIF-1αmRNA 蛋白表达的影响[J].,2011,11(10):1861-1864 |
| 生半夏、南星水提物对人胃癌BGC823 细胞的侵袭力及
HIF-1αmRNA 蛋白表达的影响 |
| Effects of Aqueous Extract of Chinese Medicine Raw Pinellia and Nanxingon Human Gastric Cancer Cells BGC823 |
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| DOI： |
| 中文关键词: 生半夏 南星 胃癌 侵袭 HIF-1α |
| 英文关键词: Raw Pinellia Nanxing Gastric cancer Attack HIF-1α |
| 基金项目: |
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| 摘要点击次数: 758 |
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| 中文摘要: |
| 目的：观察生半夏、南星中药水提物对缺氧环境中人胃癌细胞株BGC823 细胞HIF-1α 蛋白表达和侵袭力的影响。方法：运
用CoCl2（氯化钴）诱导BCG823 细胞缺氧，使得细胞中HIF-1α 蛋白表达升高. 实验组加入生半夏、南星水提物对细胞进行预处
理，然后在进行缺氧诱导。通过甲基噻唑基四唑法（MTT）检测细胞活性，使用Transwell 检测细胞侵袭能力变化，RT-PCR、Western
blotting 分别检测HIF-1αmRNA 及蛋白含量及变化。结果：生半夏、南星水提物能抑制人胃癌BGC823 细胞的增殖；生半夏、南
星水提物均能抑制缺氧诱导胃癌细胞的侵袭力，并且能降低HIF-1αmRNA 及蛋白表达。结论：生半夏、南星水提物可抑制人胃癌
BGC823 细胞的增殖，抑制人胃癌BGC823 细胞侵袭力，可能通过降低HIF-1α 蛋白表达有关。 |
| 英文摘要: |
| Objective: To investigate the effects of the enthanol and nanxing extract from Rhizome Pinelliae Preparata on hypoxiainducible
-1 alpha protein expression and aggressive influence in human gastric cancer cells BGC823. Methods: CoCl2 (chlorinated cobalt)
was used to induce BCG823 cytopathic hypoxia approach, which leads the increasing the expression of hypoxia-inducible -1 alpha.
The cell viability was detected by MTT chromatometry and cell growth curve was generated; Transwell small room was used to detect
cells attack ability, RT-PCR, Western blotting was use to detect hypoxia-inducible-1 alpha mRNA expression and protein level. Results:
The extraction of enthanol and nanxing can inhibit the proliferation of cells BGC823 human cancer;.The enthanol and nanxing extract
can inhibit the aggressive ability of cells BGC823 human cancer cells, the cause of which may be the decreasing of the expression of
HIF-1 alpha mRNA and protein. Conclusion：Enthanol extract from Rhizome Pinelliae Preparata can inhibit the BGC823 cells proliferation
and Restrain BGC823 human cancer cells aggressie influence by lowering hypoxia-inducible - 1 alpha mRNAand the protein
expression. |
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