| 王丙剑1 白剑2 孙静娴1 张娜2 徐标1△.EDU 免疫荧光法检测整合素连接激酶（ILK）高表达
对新生大鼠心肌细胞DNA 合成的影响[J].,2011,11(10):1858-1860 |
| EDU 免疫荧光法检测整合素连接激酶（ILK）高表达
对新生大鼠心肌细胞DNA 合成的影响 |
| Detection of Over-Expressed Integrin-Linked Kinase in DNA Synthesis ofCardiomyocytes in Newborn Rats by EDU Immunofluorescence Assay |
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| DOI： |
| 中文关键词: 整合素连接激酶 心肌细胞 增殖 DNA 合成 EDU |
| 英文关键词: Integrin-linked kinase Cardiomyocyte proliferation DNA synthesis EDU |
| 基金项目:国家自然科学基金项目（81070195） |
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| 中文摘要: |
| 目的：通过EDU 免疫荧光法观察整合素连接激酶（Integrin-Linked Kinase，ILK）在新生大鼠心肌细胞内高表达后对心肌细胞
DNA 合成的影响。方法：取新生（1-3 天内）大鼠原代心肌细胞，培养72 小时后随机分为正常对照组、ILK 转染组。对照组转染重组
腺病毒载体（adeno-GFP），ILK 组转染重组腺病毒载体+ILK 基因（adeno-ILK）。转然成功后48 小时将两组心肌细胞分别通过5-
乙基-2'- 脱氧尿嘧啶核苷（EDU）免疫荧光法测定心肌细胞DNA 合成。结果：ILK 转染组心肌细胞内DNA 合成较对照组明显增
加（P＜0.05）。结论：ILK 高表达具有促进新生大鼠心肌细胞的DNA 合成的能力。 |
| 英文摘要: |
| Objective: To investigate the effects of over-expressed integrin-linked kinase on rat neonatal DNA synthesis of
cardiomyocytes by EDU immunofluorescence assay. Methods: Primary neonatal rat (1-3 days old) cardiomyocytes were cultured in vitro
and randomly divided into two groups 72 h after isolation: infected by either adeno-GFP (control group) or adeno-GFP containing
adeno-ILK (ILK group). 48h after infection, DNA synthesis of cardiomyocytes in two groups were determined by 5-ethynyl-2-deoxyuridine(
EdU) immunofluorescence assay. Results: Compared with the control groups, DNA synthesis of cardiomyocytes increased dramatically
in ILK groups (P＜0.05). Conclusion: The over-expression of ILK could promote Rat Neonatal DNA synthesis of cardiomyocytes. |
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