| 魏振1 魏品康2△ 巨大维2 张慈安2 周伟2.消痰散结方对人胃癌MNK-45 荷瘤鼠机体HK-Ⅱ表达的影响[J].,2011,11(10):1841-1843 |
| 消痰散结方对人胃癌MNK-45 荷瘤鼠机体HK-Ⅱ表达的影响 |
| Effects of Eliminating Phlegm Recipe on HK-Ⅱ Expression of MNK-45Human Gastric Tumor-bearing Mice |
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| DOI： |
| 中文关键词: 消痰散结方 肿瘤 糖酵解 小鼠 |
| 英文关键词: Xiaotan Sanjie Recipe Tumor Glycometabolism Mice |
| 基金项目: |
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| 摘要点击次数: 688 |
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| 中文摘要: |
| 目的：观察消痰散结方对人胃癌MNK-45 荷瘤鼠糖酵解相关蛋白己糖激酶-Ⅱ（HK-Ⅱ）表达的影响，探讨该方抗肿瘤机理。
方法：30 只小鼠随机分为生理盐水组、消痰散结方组和希罗达组，每组10 只。建立人胃癌MNK-45 皮下移植瘤模型。灌胃治疗六
周后，称取各组瘤质量，计算抑瘤率和瘤体积。酶联免疫吸附测定法检测荷瘤鼠血清中HK-Ⅱ的水平；免疫组化方法检测瘤组织
中HK-Ⅱ蛋白的表达；结果：消痰散结方可明显抑制裸鼠人胃癌MNK-45 皮下移植瘤生长，下调荷瘤鼠血清及瘤组织中HK-Ⅱ表
达水平。结论：降低荷瘤鼠血清和组织中HK-Ⅱ的表达，抑制荷瘤鼠机体糖代谢过程可能是该方抗肿瘤作用的机制之一。 |
| 英文摘要: |
| Objective: To explore the antitumor effect of eliminating phlegm recipe on MNK-45 human gastric tumor-bearing
mice. Methods: Thirty Kunming mice were randomly divided into normal saline (NS) group, Xiaotan Sanjie Recipe group and Xeloda
group, ten for ench.Tumor-bearing models were established by injecting human gastric cancer MKN-45 injected into the subcutaneous
site of BALB/c/-nu/nu mice. The mice were administered with medicine for six weeks. Weight of tumor xenografts were measured.
tumor inhibiton rate and tumor volume were calculated. The expression of HK-Ⅱ was tested by enzyme-linked immunosorbent assay
and immunohistochemistry. Results: Xiaotan sanjie Recipe group significantly inhibits the growth of the transplanted tumor and decrease
The expression of HK-Ⅱin blood and tumor tissue. Conclusion: Xiaotan sanjie Recipe Could decrease the expression of HK-Ⅱin blood
and tumor tissue,It may be one of the mechanisms of its in inhibiting the tumor growth. |
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