| 张建民1 施晓雷2 陶科1 潘军平1 吴亚夫2.直接注射法制作ICR 小鼠肝癌原位移植瘤模型[J].,2011,11(9):1680-1682 |
| 直接注射法制作ICR 小鼠肝癌原位移植瘤模型 |
| Making an Orthotopic Tumor Model of Hepatocellular Carcinoma forICR Mice by Direct Injection |
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| DOI： |
| 中文关键词: ICR 小鼠 癌，肝细胞 模型，动物 |
| 英文关键词: ICR Mouse Carcinoma, Hepatocellular Models, Animal |
| 基金项目:南京市卫生局医学科技发展项目(ZKX09027) |
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| 中文摘要: |
| 目的：培养鼠肝癌H22 细胞，直接注射法制作ICR 小鼠肝癌原位移植瘤，为后续实验奠定基础。方法：鼠肝癌H22 细胞体外
培养，将调整好的对数生长期的肝癌细胞直接注射小鼠肝脏，2 周后解剖观察，并进行组织HE 染色。结果：所有实验小鼠均可见
肿瘤生长，HE 染色示肝细胞肝癌。结论：直接注射法制作ICR 小鼠肝癌原位移植瘤模型简便易行，值得推广应用。 |
| 英文摘要: |
| Objective: To cultivate H22 cell line and making an orthotopic tumor model of hepatocellular carcinoma on ICR
mouse using direct injection method to lay the foundation for subsequent experiment. Methods: Cultivate H22 cell line in vitro and an
orthotopic xenograft tumor model of hepatocellular carcinoma was created by injection of H22 cells in logarithmic growth phase directly
into the liver parenchyma of ICR mice. Two weeks later, hepatocellular carcinoma specimens of animals were observed and stained with
hematoxylin/eosin. Results: Tumor growth happened on all the experimental mice and was showed as hepatocellular carcinoma by
hematoxylin-eosin staining. Conclusion: It is convenient of direct injection method on making an orthotopic tumor model of
hepatocellular carcinoma and should be reported and used widely. |
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