佟丽张彩平龙石银乔新惠黄良珠高细强田英.二苯乙烯苷抑制过氧化氢诱导的人脐静脉内皮细胞凋亡[J].,2011,11(9):1675-1679 |
二苯乙烯苷抑制过氧化氢诱导的人脐静脉内皮细胞凋亡 |
Effects of TSG on Apoptosis of HUVECs Induced by H2O2 |
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DOI: |
中文关键词: 二苯乙烯苷 人脐静脉内皮细胞 细胞凋亡 H2O2 |
英文关键词: TSG HUVECs Apoptosis H2O2 |
基金项目:国家自然科学基金项目(30800474),湖南省教育厅项目(08B065, 09C832),湖南省科技厅项目(2010SK3037),
湘科条字[2009]130-19)资助 |
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中文摘要: |
目的:研究二苯乙烯苷(TSG)对过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVECs)凋亡的保护作用。方法:运用四甲基偶氮
唑盐还原法(MTT 法)和流式细胞术筛选建立细胞凋亡模型的H2O2 合适浓度以及检测不同浓度TSG 对H2O2 诱导HUVECs 的增
殖率和凋亡率;Hoechst33258 染色观察细胞凋亡形态。结果:MTT 及流式法筛选300μmol/L 为H2O2 作用于细胞的最适凋亡浓
度。MTT 和流式结果显示,与H2O2 ( 300μmol/L)损伤组比较,10μmol/L 与100μmol/L TSG 预处理组细胞的增殖率增加(P<
0.05),凋亡率显著降低(P<0.01);Hoechst33258 染色观察TSG 能降低H2O2 诱导的细胞凋亡,使细胞凋亡数减少。结论:TSG 能
抑制H2O2 诱导的HUVECs 凋亡,从而起到保护血管内皮细胞的作用。 |
英文摘要: |
Objective: To investigate the protective effect of 2,3,5,4'-tetrahydroxystilbene-2- O-β -D-glucoside (TSG on human
umbilical vein endothelial cells (HUVECs) apoptosis induced by H2O2. Methods: HUVECs were treated with H2O2
(100,200,300,400,500μmol/L) for 24 hours, the optimal concentration of H2O2 was selected by MTT and Flow Cytometry. HUVECs
were treatedwithTSG (0. 1, 1, 10, 100μmol/L) for 24 hours then exposed toH2O2 ( 100,200,300,400,500μmol/L) for 24 hours, cell viability
rates and apoptosis rates of different concentration of TSG pretreatment group were detected by MTT and Flow Cytometry. Mophology
of apoptosis and the protective effect of TSG on HUVECs induced by H2O2 were detected by Hoechst33258 staining. Results: The optimal
concentration for H2O2 to establish apoptosis model were 300μmol/L. Cell viability rate of TSG pretreatment group (10μmol/L and
100μmol/L) increased (P<0.05) while the apoptosis rate de- creased obviously (P<0.01) compared with H2O2 group(300μmol/L).
TSG reduced the number of HUVECs apoptosis. Conclusions:TSG may have a protective effect on HUVECs apoptosis induced by H2O2. |
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