| 王建勋2 初大可2 徐春盛2 张健1 王为忠2.抑癌候选基因NDRG2 对结肠癌细胞SW620 的迁移和侵袭力的影响[J].,2011,11(9):1605-1608 |
| 抑癌候选基因NDRG2 对结肠癌细胞SW620 的迁移和侵袭力的影响 |
| Effects of Candidate Tumor Suppressor Gene NDRG2 on Migratory andInvasive Ability of Colon Cancer Cells |
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| DOI： |
| 中文关键词: NDRG2 结肠癌 划痕试验 transwell 细胞侵袭实验 |
| 英文关键词: NDRG2 SW620 cell Colon cancer Transwell invasion Wound healing |
| 基金项目:国家863 高技术研究发展计划(2006AA02Z194)；国家自然科学基金(30700416，30672074) |
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| 中文摘要: |
| 目的：观察NDRG2 对结肠癌SW620 细胞侵袭、转移等生物学行为的影响，探讨其可能的调节机制。方法：用阳离子脂质体
转染方法分别转染pcDNA3.1-Ndrg2 和SiRNA-Ndrg2 于SW620 细胞内48h，上调/ 下调NDRG2 的表达；检测NDRG2 基因mRNA
及蛋白表达水平的变化；通过划痕试验及transwell 细胞侵袭试验进一步对上调/ 下调NDRG2 表达水平后的结肠癌细胞迁移和
侵袭能力进行分析。结果：pcDNA3.1-Ndrg2 转染SW620 后，NDRG2 的mRNA 和蛋白表达水平明显升高，细胞的迁移和侵袭能
力下降；SiRNA-Ndrg2 转染SW620 后，NDRG2 的mRNA 和蛋白表达水平明显降低，细胞的迁移和侵袭能力上升，差异具有统计
学意义（P＜0.05）。结论：NDRG2 作为抑癌候选基因能够降低结肠癌细胞转移和侵袭能力。 |
| 英文摘要: |
| Objective: To observe the influence of NDRG2 on the migratory and invasive ability of human colon cancer cell line
SW620. Method: pcDNA3.1-Ndrg2 and SiRNA-Ndrg2 were transfected transiently respectively into SW620 by Lipofectamine TM 2000.
Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were used to investigate the expression of mRNA and protein
of NDRG2. Matrigel invasion assays and wound - healing experiment were used to study the movement and invasion in human colon
cancer cell line SW620. Result: After transfecting pcDNA3.1-Ndrg2 into SW620 cells, the mRNA and protein level of NDRG2 increased
markedly and the ability of migration and invasion decreased significantly. After transfeting SiRNA-Ndrg2 into SW620 cells, the mRNA
and protein level of NDRG2 declined markedly and the ability of migration and invasion increased significantly. Conclusion: NDRG2 as
a candidate tumor suppressor gene can reduce the ability of metastasis and invasion of colon cancer cells. |
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